【作者】 柯碧霞；

【Author】 Ke Bixia;Guangdong Provincial Center for Disease Controf and Prevention;

【机构】 广东省疾病预防控制中心病原微生物检验所；

【摘要】 目的了解广东省人源沙门菌1,4,[5],12:i:-的流行现状、耐药性与分子特征。方法对广东省2007-2016年腹泻病人沙门菌监测检出的2960株沙门菌1,4,[5],12:i:-进行药物敏感试验、PCR检测和脉冲场凝胶电泳(PFGE)分型。结果 2007-2016年广东省共检出人源沙门菌1,4,[5],12:i:-2960株,检出率逐年增多,至2015年成为广东省人源沙门菌中最主要的血清型。男女性别比例为1.58:1,发病年龄以婴幼儿为主。沙门菌1,4,[5],12:i:-除对亚胺培南100%敏感外,对其它17种抗生素均有不同程度的耐药。2011年-2016年对头孢他啶、头孢噻肟和环丙沙星的耐药率呈上升趋势。多重耐药现象严重,具有ASSuT多重耐药的有70.62%(435/616),具有ACSuGSTTm多重耐药的有27.11%(167/616)。第Ⅱ相鞭毛蛋白不表达主要(75.53%)是缺失fljA、fljB和hin基因,但保留了iroB、STM2740、STM2757基因,共有8种不同的缺失方式。2347株菌有934种不同PFGE谱型别,表现出较大的指纹图谱多态性,最主要的优势PFGE谱型为JPXX01.GD0226型,有97株菌,占4.13%(97/2347)。168种沙门菌1,4,[5],12:i:-的PFGE图谱与鼠伤寒沙门菌PFGE图谱是一致的。结论随着沙门菌1,4,[5],12:i:-的检出不断增多,其已成为广东省人源沙门菌中最主要的血清型。沙门菌1,4,[5],12:i:-的多重耐药现象严重,第Ⅱ相鞭毛蛋白不表达主要由缺失fljA、fljB和hin基因引起,其PFGE型别多样,呈遗传多态性。更多还原

【Abstract】 Objeetive To understand the circulation, drug resistance and molecular characteristics of Salmonella 1,4,[5],12:i:-in human in Guangdong province.Methods Salmonella 1,4,[5],12:i:-isolated from diarrhea patients in Guangdong during 2007-2016 were detected for drug resistance,genes and PFGE characteristics.Results A total of 2 960 strains Salmonella 1,4,[5],12:i:-were isolated from human diarrhea cases during this period.The positive rates of the isolation increased year by year.The male to female ratio of the infection cases was 1.58:1 and the infection mainly occurred in infants and young children.Except imipenem,Salmonella 1,4,[5],12:i:-was resistant to other 17 antibiotics to some extent. The drug resistant rates to ceftazidime, cefataxime and ciprofloxacin increased from 2011 to 2016.Multi-drug resistance was serious,for example,the multi-drug resistant strains with ASSuT accounted for 70.62%(435/616) and the multi-drug resistant strains with ACSuGSTTm accounted for 27.11%(167/616).Lack of flja,fljB and hin genes,as well as the retaining of iroB,STM2740,STM2757 genes,resulted in the unable expression of FljBenx gene with 8 different defection profiles.There were 934 different PFGE patterns observed in 2347 strains. which displayed a relatively large fingerprint polymorphism.The major PFGE pattem was.mXX01.GD0226,which Was found in 97 strains,accounting for 4.13%(97/2347).The PFGE patterns in 168 Salmonella 1,4,[5],12:i:-strains were consistent with that of Salmonella typhimurium.Conclusion Salmonella1,4,[5],12:i:-strains has become the major serotype of Salmonella that cause diarrhea in human in Guangdong.The multi.drug resistance of Salmonella 1,4,[5],12:i:-was serious,and since the defection of fljA,fljB and hin genes,the expression of FljBenx protein failed.The PFGE results were diverse,which displayed polymorphism in inheritance.更多还原