【作者】 董爽爽； 王利彬； 程敦公； 任勇； 张业伦； 穆平； 张玉梅； 刘建军； 李豪圣； 赵振东； 郝元峰； 曹新有；

【Author】 Dong Shuang-shuang;Wang Li-bing;Cheng Dun-gong;Ren Yong;Zhang Ye-lun;Mu Ping;Zhang Yu-mei;Liu Jian-jun;Li Hao-sheng;Zhao Zhen-dong;Hao Yuan-feng;Cao Xin-you;College of Agronomy,Qingdao Agricultural university;Crop Research Institute,Shandong Academy of Agricultural Sciences/National Engineering Laboratory for Wheat and Maize/Key Laboratory of Wheat Biology and Genetic Improvement in North Yellow and Huai River Valley,Ministry of Agriculture;Mianyang Institute of Agricultural Sciences;Institute of Cereal and Oil Crops,Hebei Academy of Agriculture and Forestry Sciences;Institute of Crop Science,Chinese Academy of Agricultural Sciences;

【机构】 青岛农业大学农学院； 山东省农业科学院作物研究所/小麦玉米国家工程实验室/农业部黄淮北部小麦生物学与遗传育种重点实验室； 四川省绵阳市农业科学研究院； 河北省农林科学院粮油作物研究所； 中国农业科学院作物科学研究所；

【摘要】 小麦成熟期对粮食周年丰产具有重要的决定作用。为了给小麦分子标记辅助育种提供可用的分子标记,本研究以人工合成六倍体小麦（Bubo）和T.spelta L.衍生系（Turtur）为亲本创制的包含186个家系的RIL群体（F₆）为材料,构建了包含5301个标记（4120个DarT标记、621个SNP标记和560个传统DarT标记）,总长为2464cM的遗传连锁图谱,利用Windows QTL Cartographer2.5软件的复合区间作图法对在3年4点的环境下的成熟期性状进行QTL检测,在LOD>2.5水平下,共定位到15个QTL位点,分布于小麦的1A、2B、2D、3A、4A、4B、5B、7A和7B染色体上,可解释4.42%～12.67%的表型变异。其中在1 A染色体上控制小麦成熟期的QTL贡献率最大;4B染色体的1215714～1068877F0-44CG区间内3年3点均检测到的QTL与11215714标记遗传距离为0.01cM,近乎共分离,为下一步分子标记辅助选择的精准性提供了坚实的基础。更多还原

【Abstract】 The maturity of wheat has an important role in determining the annual grain yield of grain.In order to provide molecular marker for wheat molecular marker-assisted breeding,a population of 186 F₆ RILs derived from a cross between Triticum spelta L.（Turtur） and synthetic hexaploid wheat（Bubo） was used to detect the QTL for maturitv.A high-density genetic map was constructed,which includes 5301 markers（4120 Silico DarT,621 SNPs and 560 traditional DarT array markers） and spanning about2464 cM.Using the field data in three years and four environment,QTLs associated with maturity were detected using composite interval mapping（CIM） method in Windows QTL Cartographer 2.5 software.Under the level of LOD>2.5,a total of 15 QTLs were detected in 1 A、 2 B、 2 D、 3 A、 4 A、 4 B、 5 B、 7 A and 7 B chromosomes.These QTLs could explain 4.42%¹2.67%of phenotypic variance.Among them,the QTL on 1 A chromosomes has biggest contribution rate.In the interval between 1215714 and1068877 F0-44 CG on chromosome 4 B,the QTL were only 0.01 cM away from 1215714 marker,almost were co-separated,which lays a solid foundation for the accuracy of molecular marker assisted selection.更多还原