【作者】 王立国； 傅明川； 李浩； 刘任重； 柳展基；

【Author】 Wang Liguo;Fu Mingchuan;Liu Renzhong;Liu Zhanji;Key Laboratory of Cotton Breeding and Culitvation in Huang-Huai-Hai Plain,Ministry of Agriculture,Shandong Cotton Research Center;

【机构】 农业部黄淮海棉花遗传改良与栽培生理重点实验室/山东棉花研究中心；

【摘要】 NAC基因家族是植物中特有的一类转录因子,其功能涉及植物生长发育调控、激素信号转导、逆境胁迫响应等多种生理过程。本研究从陆地棉中克隆了一个NAC基因GhSNAC1,长度为1104bp,推测编码蛋白含有299个氨基酸,分子量为33.9 kDa,等电点为6.18。蛋白序列分析表明,GhSNAC1具有典型的NAC转录因子特征,其N端含有保守的NAC结构域。实时荧光定量PCR结果显示,GhSNAC1受到干旱、高盐、低温和黄萎病菌胁迫诱导。异源表达转基因功能初步分析表明,过量表达GhSNAC1的转基因烟草抗旱耐盐能力显著提升。更多还原

【Abstract】 NAC genes,plant-specific transcription factors,play crucial roles on developmental regulation,hormone signal transduction,biotic and abiotic stress responses.In the present study,a NAC gene,named as GhSNAC1,was isolated from upland cotton(Gossypium hirsutum L.).The sequence analysis indicated that GhSNAC1 was 1104 bp long and encoded a protein of 299 amino acids with a relative molecular mass of 33.9 kDa and an isoelectric point of 6.18.Expression analysis showed GhSNAC1 was induced by drought,salt,cold and Verticillium wilt.Preliminary analysis of ectopic gene expression suggested that overexpression of GhSNAC1 in tobacco enhanced tolerance to drought and salt stresses.更多还原