【作者】 胡日生； 朱咸鑫； 向世鹏； 阳成伟； 赖建彬； 张先文； 刘志； 朱列书；

【Author】 Risheng Hu;Xianxin Zhu;Shipeng Xiang;Chengwei Yang;Jianbin Lai;Xianwen Zhang;Zhi Liu;Lieshu Zhu;Central South Agricultural Experiment Station of China Tobacco;Hunan Agricultural University;South China Normal University;

【机构】 中国烟草中南农业试验站； 湖南农业大学； 华南师范大学；

【摘要】 低温是影响烟草生长发育的主要环境胁迫之一,对烟叶生产有重要影响。为揭示烟草耐冷性的分子机制,我们采用蛋白组学iT RAQ技术对两个耐冷不同的烟草品种NC567和台烟8号进行了研究。共鉴定出4317个差异蛋白,并对四个比较组获得的差异蛋白进行了深入分析。GO分析显示大部分差异蛋白参与代谢和细胞加工过程。在低温条件下,有55个蛋白在NC567中下调表达,但在台烟8号中上调表达。台烟8号中42个蛋白在常温条件下较低水平表达,而低温处理时的表达水平要高于NC567。研究结果揭示了这两个烟草品种在冷胁迫响应上的差异。这些差异表达蛋白涉及蛋白质合成和降解、光合作用和呼吸作用以及活性氧(ROS)清除等,它们对细胞低温响应过程中内在环境的建构具有重要作用。本研究鉴定的低温胁迫相关蛋白和代谢途径对烟草耐低温改良具有指导意义。更多还原

【Abstract】 Given the development of tobacco is sensitive to low temperature, cold stress is a critical problem for tobacco production. Uncovering of the mechanisms for cold response in tobacco would contribute to improving its cold tolerance, but the precise molecular mechanism in this process remains unclear. In our novel screening for cold sensitivity in different tobacco cultivars, NC567 displayed a cold tolerance phenotype, while Taiyan8 is hypersensitive to low temperature. In the current study, the samples of NC567 and Taiyan8, with or without cold treatment, were used in i TRAQ based proteomics to uncover their different mechanisms in response to cold stress. A total of 4317 distinct proteins were identified and the differential proteins in four comparison sets were used for further analysis. The GO analysis indicated that the majority of differentially expressed proteins were involved in metabolic and cellular processes. There were 55 proteins decreased in NC567 but increased in Taiyan8 under low temperature, while the levels of 42 proteins were lower in Taiyan8 at normal temperature but higher in Taiyan8 than NC567 under cold treatment, suggesting the variations for cold response in these cultivars. The levels of components involved in protein synthesis/degradation, photosynthesis/respiration, as well as ROS scavenging, were significantly different in these comparison sets, providing evidence for a model that protein metabolism and energy balance are essential for creation of an intracellular environment in response to low temperature in tobacco cells. In conclusion, our study identified several novel pathways associated to low temperature stress and provides hints for the further improvement of cold tolerance in tobacco plants.更多还原