【作者】 朱邦勇； 李伟； 段家俊； 张杰； 韦江平； 周志光； 黄耿； 唐中书； 陈羽建； 曹娟； 甘泉； 黄寅杰；

【Author】 ZHU Bang-yong;LI Wei;DUAN Jia-jun;ZHANG Jie;WEI Jiang-ping;ZHOU Zhi-guang;HUANG Geng;TANG Zhong-shu;CHEN Yu-jian;CAO Juan;GAN Quan;HUANG Yin-jie;Institute of Dermatology,Guangxi Autonomous Region;

【机构】 广西壮族自治区皮肤病防治研究所；

【摘要】 目的为了解广西部分地区梅毒螺旋体的基因分型状况。方法从2012年1月～2016年7月在广西地区的性病门诊收集疑似早期梅毒患者300例,采集组织液,采用镀银染色法检测梅毒螺旋体,同时采用PCR扩增TP polA基因,再进行TP的arp基因60个碱基对重复序列的数目、tprⅡ基因MseⅠ酶切后限制性片段长度多态性的型别和tp0548基因序列的型别进行鉴别,依据上述结果进行综合分析以判断梅毒基因型,统计学资料采用SPSS13.0软件进行分析。结果在215例确诊的早期梅毒患者中,镀银染色法检测出105例,阳性率为48.8%,梅毒PCR检测阳性标本210例,阳性率为97.7%。在PCR阳性标本中,进行三基因完全分型数为190例,总计分出17个基因型别,14d/f占86例(45.3%),具有绝对的流行优势,其他亚型包括15d/f(26)、16d/f(22)、17d/f(14)、13d/f(13)、10d/f(8)、18d/f(3)、16a/f(3)、5d/f(2)、7d/f(2)、12d/f(2)、16d/e(2)、14a/f(2)、9h/c(2)、15l/f(1)、25a/e(1)、15i/f(1)。结论镀银染色法检测早期梅毒在基层实验室具有推广价值,广西地区早期梅毒患者的梅毒螺旋体基因型别具有多样性,其中以14d/f型为优势型。更多还原

【Abstract】 Objective To understand genotyping of treponema pallidum,and to provide data on the epidemic of syphilis in Guangxi.Methods We enrolled suspected 300 cases of early syphilis in Guangxi STD clinics from January 2012 to July 2016 year,collected tissue fluid of patients,and detected treponema pallidum by Silver staining method,and performed nested PCR for samples.PCR was used to apply TP polA gene,and then the umber of repeated base pairs in arp gene,the type of tpr Ⅱgene after restriction fragment length by Mse Ⅰenzyme polymorphism,and tp0548 gene type were comprehensively analyzed for the genotype of the treponema pallidum stain.Results Out of 215 patients with early syphilis,105 cases(48.8%) were positive by Silver staining method and 210 cases were positive(97.7%) for PCR.Of 190 TP-positive specimens genotyped by the three genes method,17 genotypes were found,14 d/f(86,45.3%) were predominant,other including 15 d/f(26),16 d/f(22),17 d/f(14),13 d/f(13),10 d/f(8),18 d/f(3),16 a/f(3),5 d/f(2),7 d/f(2),12 d/f(2),16 d/e(2),14 a/f(2),9 h/c(2),15 l/f(1),25 a/e(1) and 15 i/f(1).Conclusion Application of treponema pallidum detection in early syphilis by silver impregnation in primary laboratories.Treponema pallidum genotypes were diversified in early syphilis patients in guangxi region,and 14 d/f were predominant.更多还原