【作者】 张引； 张祝兰； 邱观荣； 杨煌建； 王德森； 任林英； 连云阳；

【Author】 Zhang ying;Zhang Zhu-lan;Qiu Guan-rong;Yang Wang-jian;Wang De-sen;Ren Lin-ying;Lian Yun-yang;Fujian Provincial Key Laboratory of Screening for Novel Microbial Products,Fujian Institute of Microbiology;

【机构】 福建省微生物研究所福建省新药(微生物)筛选重点实验室；

【摘要】 替考拉宁产生菌替考游动放线菌（Actinoplanes teichomycoticus）FIM-16经Co⁶⁰诱变处理,以替考拉宁自身抗性作为选择压力,通过摇瓶大量筛选,获得一株高产菌FIM-16-58,其摇瓶发酵水平较菌株FIM-16提高了2倍,且遗传稳定。在1T发酵罐上发酵,添加0.1%的玉米油可有效抑制泡沫的产生,替考拉宁产量5.0 g/L以上。更多还原

【Abstract】 After being treated by Co⁶⁰irradiation,spore suspension of teicoplanin producing strain Actinoplanes teichomyceticus FIM-16 was spreaded on the regenerating plate containing lethal concentration of teicoplanin.Teicoplanin-resistant mutants were picked up and screened by shake flask fermentation.Strain FIM-16-58 showed 2 times higher of teicoplanin yield than that of original strain was obtained and demonstrated high hereditary stability.During scale-up experiment in the 1 T fermentor,foams were suppressed effectively by addition of 0.1%corn oil,and the yield of teicoplanin reached more than 5.0 g/L.更多还原