【作者】 杨煌建； 张祝兰； 唐文力； 任林英； 王德森； 连云阳；

【Author】 Yang Huang-jian;Zhang Zhu-lan;Tang Wen-li;Ren Lin-ying;Wang De-sen;Lian Yun-yang;Fujian Key Laboratory of Screening for Novel Microbial Products,Fujian Institute of Microbiology;

【机构】 福建省微生物研究所福建省新药(微生物)筛选重点实验室；

【摘要】 目的:探讨红色诺卡氏菌细胞壁骨架(N-CWS)对小鼠皮肤创伤愈合的影响。方法:建立小鼠背部皮肤创伤模型,通过N-CWS冻干粉涂布给药,观察小鼠伤口愈合时间和愈合率,HE染色观察创面组织学的变化,免疫组织化学染色法检测血管内皮生长因子(VEGF)和血小板源性生长因子(PDGF)的动态改变。结果:N-CWS能明显提高小鼠创伤愈合速度和愈合率(与空白组比较,P<0.05或P<0.01)。HE染色显示实验组毛细血管、胶原纤维增生明显,表皮再生完全。免疫组织化学染色显示,实验组在5d时VEGF表达水平明显高于空白组(P<0.05),实验组在7d时PDGF表达水平明显高于空白组(P<0.05)。结论:N-CWS在创伤修复愈合过程中起明显促进作用,可能与调节VEGF和PDGF生长因子表达有关。更多还原

【Abstract】 Objective To study the influence of Nocardia rubra cell wall skeleton(N-CWS)on wound healing of mouse skin.Methods The mouse skin wound model was established.Mouse wound healing time and healing rate were recorded after treatment with N-CWS.The change of wound histology was observed by hematoxylin-eosin(HE)staining and the dyanmic change of vascular endothelial growth factor(VEGF) and platelet-derived growth factor(PDGF) were detected by immunohistochemical staining method respectively.Results Compared with blank group,N-CWS could obviously improve the healing speed and healing rate of wound in mouse(P<0.05 or P<0.01).HE staining revealed that remarkable capillary and collagen fiber hyperplasia,and complete epidermal regeneration was observed in experimental group.Immunohistochemical staining revealed that the expression level of VEGF in experimental group was significantly higher than that of blank group in 5 d(P <0.05).The expression level of PDGF was greatly higher than that of blank group in 7 d(P <0.05).Conclusion N-CWS plays a significant promotion role in healing and repair of wound,which could be related to promoting the expression of VEGF and PDGF.更多还原