【作者】 陶易凡； 强俊； 殷国俊； 徐跑； 石琼； 包景文；

【Author】 Yi-Fan Tao;Jun Qiang;Guo-Jun Yin;Pao Xu;Qiong Shi;Jin-Wen Bao;Wuxi Fisheries College, Nanjing Agricultural University;Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences;Shenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI;

【机构】 南京农业大学无锡渔业学院； 中国水产科学研究院淡水渔业研究中心农业部淡水渔业与种质资源利用重点实验室； 深圳市海洋生物基因组学重点实验室；

【摘要】 为了解miRNA在吉富罗非鱼(GIFT,Oreochromis niloticus)肝脏脂代谢中的作用,本研究分别构建了高脂饲养和正常饲养罗非鱼肝脏小RNA文库,采用高通量测序技术进行测序。利用生物信息学方法在两文库中共筛选到204个已知miRNA和56个新型miRNA。通过荧光定量PCR验证发现有6个脂代谢相关miRNA(miR-30a-5p,miR-34a,miR-145-5p,miR-29a,miR-205-5p,miR-23a-3p)在高脂组和正常组间存在差异表达。这些差异表达miRNA靶基因预测和功能注释表明它们在调控罗非鱼脂代谢中发挥了重要的作用。本研究为进一步探究miRNA在罗非鱼脂质代谢调控中的作用奠定了基础,为减少罗非鱼脂肪肝病发生提供了新思路新靶点。更多还原

【Abstract】 To investigate the miRNAs involved in GIFT lipid metabolism, two hepatic small RNA libraries from high-fat diet-fed and normal-fat diet-fed GIFT were constructed and sequenced using high-throughput sequencing technology. A total of 204 known and 56 novel miRNAs were identified by aligning the sequencing data with known Danio rerio miRNAs listed in miRBase 21.0. Six known miRNAs(miR-30a-5p, miR-34 a, miR-145-5p, miR-29 a, miR-205-5p, and miR-23a-3p) that were differentially expressed between the high-fat diet and normal-fat diet groups were validated by quantitative real-time PCR. Bioinformatics tools were used to predict the potential target genes of these differentially expressed miRNAs, and Gene Ontology enrichment analysis indicated that these miRNAs may play important roles in diet-induced hepatic steatosis in GIFT. Our results provide a foundation for further studies of the role of miRNAs in tilapia lipid homeostasis regulation, and may help to identify novel targets for therapeutic interventions to reduce the occurrence of fatty liver disease in farmed tilapia.更多还原