【作者】 史健志； 王文磊； 许凯； 徐燕； 纪德华； 陈昌生； 谢潮添；

【Author】 SHI Jianzhi;WANG Wenlei;XU Kai;XU Yan;JI Dehua;CHEN Changsheng;XIE Chaotian;College of Fisheries, Jimei university;

【机构】 集美大学水产学院；

【摘要】 坛紫菜自然生长于潮间带地区,伴随着潮汐涨落需要经历周期性的失水和复水过程,其细胞如何适应失水逆境环境,目前仍然缺乏一个全面的解释。本研究通过综合分析坛紫菜叶状体细胞在不同失水程度(0%,30%,60%,90%)以及复水下的转录组和蛋白质组变化,初步解析了其耐受失水胁迫的分子调控机理。在转录组水平,采用RNA-seq技术鉴定了7263条差异基因(F≥2);在蛋白质组水平,采用i TRAQ技术鉴定了303个差异蛋白(F≥1.2),并通过比较转录组和蛋白质组数据初步揭示了坛紫菜耐受失水过程的代谢调控过程。首先,受失水胁迫影响,一些基本的生命过程受到抑制,如光合作用,次级代谢,蛋白质合成等;其次,细胞在失水过程中,通过合成脂类以及细胞骨架,激活一系列的防御响应(分子伴侣,抗氧化系统)来维持细胞内的稳态;以上这些过程需要多种途径来提供能量,包括糖酵解,TCA循环等。此外,一些基因在转录水平和蛋白水平的表达并不一致,这表明在坛紫菜中广泛存在着转录后调控,具体机制还需要进一步的研究。本研究为深入解析坛紫菜耐受失水胁迫的调控网络奠定了基础。更多还原

【Abstract】 Pyropia haitanensis naturally grow in intertidal zone, which make it undergo periodic dehydration and rehydration processes. However, the mechanism of P. haitanensis to survive under such condition is still lacking a comprehensive explanation. In this research, we comparatively analyzed the transcriptome and proteome of P. haitanensis blades under different dehydration level(0%, 30%, 60%, 90%) stress and rehydration, preliminarily explained the molecular regulation of desiccation tolerance. At the transcript level, 7263 genes showed a 2-fold or more expression difference as detected by an RNA sequencing approach. At the protein level, 303 proteins differed by 1.2-fold or more in their relative abundance, as indicated by iT RAQ(isobaric tags for relative and absolute quantitation) analysis. The regulation processes of P. haitanensis were revealed by comparing the transcriptome and proteome. Firstly, by the impact of desiccation, some basic metabolic processes are inhibited, such as photosynthesis, secondary metabolism and protein synthesis; Secondly, in order to keep cellular homeostasis, cells increased the productions of lipids and cytoskeleton, and activated a series of defense responses, such as molecular chaperones and antioxidant system. The above energy-consumed processes were supported by glycolysis and TCA cycles. In addition, a number of genes showed inconsistency between the transcript and protein level, which is indicative of post-transcriptional events. These results provide a basis for understanding the molecular regulation of desiccation tolerance in P. haitanensis.更多还原