Genetic characterization of Toxoplasma gondii from wild rodents in Sichuan Province,Southwestern China

【作者】 王鑫磊； 董玲； 张莉； 吕艳； 李倩； 李海龙；

【Author】 Xin-Lei Wang;Ling Dong;Li Zhang;Yan Lv;Qian Li;Hai-Long Li;College of Basic Medicine,Dali University;Jinci College of Shanxi Medical University;

【机构】 College of Basic Medicine,Dali University； Jinci College of Shanxi Medical University；

【摘要】 Background:Wild rodents are the intermediate hosts of Toxoplasma gondii.The distribution of genetic diversity of T.gondii in wild rodents is of importance to understand the transmission of this parasite.The objective of present study was to genetically characterize T.gondii isolates from wild rodents in Sichuan province,southwestern China.Methods:Genomic DNA was extracted from 10 g wild rodents brain samples.Seminested PCR and multilocous polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) technology were performed to examine genetic diversity of T.gondii isolates as described previously.Results:In total,181 brain tissues of different wild rodents,including Eothenomys miletus(n=88),Crocidura attenuate(n=9),Rattus rattus sladeni(n=46),Mus musculus Linnaeus(n=6) and R.niviventer(n=32) were tested for T.gondii DNA,respectively.Six of them were found to be positive for the T.gondii B1 gene by seminested PCR amplification,4 showed complete genotyping results for all 11 polymorphic loci(SAG1,SAG2,alt.SAG2,SAG3,BTUB,GRA6,L358,PK1,C22-8,C29-2 and Apico) by PCR-RFLP,which were determined to represent a potential new genotype(http://toxodb.org/toxo/).Conclusion:These results documented genetic characterization of T.gondii in wild rodents from Sichuan province,and enriched the genetic diversity of T.gondii in China.更多还原

【Abstract】 Background:Wild rodents are the intermediate hosts of Toxoplasma gondii.The distribution of genetic diversity of T.gondii in wild rodents is of importance to understand the transmission of this parasite.The objective of present study was to genetically characterize T.gondii isolates from wild rodents in Sichuan province,southwestern China.Methods:Genomic DNA was extracted from 10 g wild rodents brain samples.Seminested PCR and multilocous polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) technology were performed to examine genetic diversity of T.gondii isolates as described previously.Results:In total,181 brain tissues of different wild rodents,including Eothenomys miletus(n=88),Crocidura attenuate(n=9),Rattus rattus sladeni(n=46),Mus musculus Linnaeus(n=6) and R.niviventer(n=32) were tested for T.gondii DNA,respectively.Six of them were found to be positive for the T.gondii B1 gene by seminested PCR amplification,4 showed complete genotyping results for all 11 polymorphic loci(SAG1,SAG2,alt.SAG2,SAG3,BTUB,GRA6,L358,PK1,C22-8,C29-2 and Apico) by PCR-RFLP,which were determined to represent a potential new genotype(http://toxodb.org/toxo/).Conclusion:These results documented genetic characterization of T.gondii in wild rodents from Sichuan province,and enriched the genetic diversity of T.gondii in China.更多还原