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东北梅花鹿线粒体COⅡ基因序列的遗传结构及其系统发育分析

Genetic structure and phylogenetic analysis of the mtDNA COⅡ gene in Northeastern sika deer

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【作者】 徐佳萍荣敏杨福合邢秀梅

【Author】 Xu JiaPing;Rong Min;Yang Fuhe;Xing Xiumei;Institute of Special Economic Animal and Plat Science,Chinese Academy of Agricultural Sciebces,State Key Laboratory of Special Economic Animal Molecular Biology,Key Laboratory of Special Economic Animal Genetic Breeding and Reproduction;

【机构】 中国农业科学院特产研究所吉林省特种经济动物分子生物学省部共建实验室特种经济动物种质资源遗传改良重点开放实验室

【摘要】 采用基因测序的方法,对3种东北梅花鹿的COⅡ基因片段进行了PCR扩增和测序。结果表明,3种东北梅花鹿COⅡ基因片段的长度均为740bp,A+T含量都大于G+C的含量。3个品种单倍型多样性较高(0.673~0.717),就单个品种而言,敖东梅花鹿的遗传多样性最高。

【Abstract】 MtDNA CO Ⅱ gene fragments of three Northeastern sika deer species were amplified using PCR method,and the products were purified and sequenced.The result showed that all the mtDNA CO Ⅱ gene fragments had 740 bp,and had greater amount of the A + T pairs than the G + C pairs.As a whole,the haplotype diversity was high(0.673 ~0.717).In a single species,aodong sika deer had a much higher genetic diversities.

【基金】 吉林省自然科学基金“利用DNA条形码评估吉林梅花鹿资源”(20101567)
  • 【会议录名称】 “十二五” 鹿科学研究进展
  • 【会议时间】2016-06
  • 【分类号】S825
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