【作者】 闫婷婷； 孟雪萍； 李文龙； 汤克勇；

【Author】 YAN Tingting;MENG Xueping;LI Wenlong;TANG Keyong;College of Materials Science and Engineering,Zhengzhou University;

【机构】 郑州大学材料科学与工程学院；

【摘要】 以新鲜猪皮为原料,采用酸酶结合法从猪皮中提取胶原蛋白。通过聚丙烯酰胺凝胶电泳(SDS-PAGE)、红外光谱(FT-IR)、紫外光谱(UV-vis)和热分析系统(TG--DSC)表征了所提取胶原蛋白的分子结构和热稳定性。研究结果表明,所提取的胶原蛋白为I型胶原蛋白,且保持完整的三股螺旋结构,其热变性温度约为70℃。采用冷冻-解冻法制备了胶原/壳聚糖/聚乙烯醇复合水凝胶敷料,并对其结构形貌、溶胀能力、保水能力、水蒸气透过率、孔隙率、拉伸强度、断裂伸长率等进行了表征。结果表明,冷冻-解冻法制备的胶原/壳聚糖/聚乙烯醇复合水凝胶呈现网孔尺寸均一的网络结构,溶胀能力和保水能力较强,孔隙率高达95%,力学性能优异,最大和最小拉伸强度分别为55.45 MPa、47.75 MPa。更多还原

【Abstract】 Collagen was extracted from fresh pigskin by the combination of acid and enzyme method and then,characterized with SDS-PAGE,FTIR,UV-vis and TG-DSC.The result clearly suggested that the extracted product was type-Ⅰ collagen with the triple helix structure and the thermal denaturation temperature of which is close to 70 ℃.Besides,the collagen/chitosan/PVA composite hydrogel was prepared by a freeze-thaw method and the structure and properties such as swelling capacity,water retention capacity,water vapor permeability,porosity,tensile strength and the elongation at break were characterized.The results indicated that the collagen/chitosan/PVA composite hydrogel has homogeneous network structure,strong swelling,good water retention capacity,and excellent mechanical properties.The porosity up to 95%,the maximum and the minimum tensile strength is 55.45 MPa and 47.75 MPa,respectively.更多还原