【作者】 桑滨； 鲁翠云； 李超； 孙效文； 李丹彤；

【Author】 SANG Bin;LU Cui-yun;LI Chao;SUN Xiao-wen;LI Dan-tong;College of Fisheries and Life, Dalian Ocean University;Heilongjiang Fisheries Research Institute, Chinese Academy of Fishery Sciences;

【机构】 大连海洋大学水产与生命学院； 中国水产科学研究院黑龙江水产研究所；

【摘要】 耐寒品种的培育与养殖对于三北地区的鲤养殖业非常重要,对育成品种进行遗传分析有助于种质资源的可持续利用。本研究采用30个微卫星标记分析了3个遗传背景相近的耐寒鲤品种松荷鲤(GG,Cyprinus carpio L.)、荷包红鲤抗寒品系(HH,Cyprinus carpio var.wuyuanensis)与松浦鲤(SS,Cyprinus carpio var.Songpu)的遗传多样性。结果显示:(1)3个耐寒鲤品种GG、HH和SS的平均多态信息含量为0.720、0.717和0.720,均属高度多态水平(PIC>0.5),3个群体偏离Hardy-Weinberg平衡的位点较少,种群结构合理。(2)基于Structure亚种群数检验的分析结果显示,当K=2时,HH群体从3个群体中分离出来,GG和SS群体主要遗传组分相同;当K=3时,GG、HH和SS群体相互分离开,并且各自形成自己的遗传组分。(3)在26个标记中检测出品种特异等位基因73个,其中41个品种特异等位基因的频率超过10%,进而获得基因型频率大于10%的品种特异基因型161个,可用于3个耐寒鲤品种的种质鉴定。本研究旨在为鲤的养殖和种质鉴定等提供可靠的参考依据。更多还原

【Abstract】 Cultivation and breeding of cold resistant varieties are very important for carp farming in the Three-North Region, meanwhile, genetic analysis of cultivars is helpful to the sustainable utilization of the germplasm resources. Comparative analysis of genetic diversity was conducted using microsatellite markers on three cold-resistant varieties of common carp with similar genetic background, Cyprinus carpio L.(GG), Cyprinus carpio var. wuyuanensis(HH), and Cyprinus carpio var. Songpu(SS).The results showed as following:(1) The average polymorphism information content of three cold-resistant varieties of common carp(GG, HH, SS) was 0.720,0.717, 0.720, respectively. Three cold-resistant varieties of common carp were of highly polymorphic level. Three populations deviated from the Hardy-Weinberg equilibrium and the population structure were reasonable.(2) In accordance with the results of Structure analysis, HH separated as a group alone, GG and SS clustered together for the model K=2. Whereas GG, HH,and SS were clustered into 3 different groups for the model K =3.(3) 73 specific alleles were detected from 26 markers, of which 41 specific alleles frequency reached more than 10%.更多还原