Hepatitis C virus core impacts expression of miR122 and miR204 to influence carcinogenic progression via down regulation of TGFBRAP1 and HOTTIP

【作者】 窦骏； 王小英； 彭杰夫； 廖继佩； 吴迪； 李淼； 吴松颜；

【机构】 东南大学医学院； 江南大学医学院； 江苏省CDC；

【摘要】 In this study,we investigated the effect of presence of hepatitis C virus(HCV) core upon the expression of IncRNAs,mRNAs,and circRNAs in human Huh7 hepatoma(Huh7) cells,and focused on the possible carcinogenic mechanism of HCV core protein.The data showed that 4851 lncRNAs,4785 mRNAs,and 823 circRNAs were 2.0-fold up-regulated in Huh7-core cells.The genes in the enriched set were associated with macromolecule metabolic processes and DNA damage response etc.By the Kyoto encyclopedia of genes and genome pathway analyses,the identified 10 genes from the selected 14 genes were higher or lower expression in Huh7-core cells than that of Huh7-vector cells analyzed by qPCR.Interestingly,overexpression of miR122 and miR204 partly abrogated the expression of TGFBRAP1 and HOTTIP,and increased the HPCAL1 expression in the predicted carcinogenic pathways.Our data suggested that the pathways of miR204-HPCAL1-lncRNAHOTTIP and miR122-TGFBRAP1 likely involved in the carcinogenic progress due to HCV core of presence,and that miR122 and miR204 overexpression might inhibit the progress by down-regulation of TGFBRAP1 and HOTTIP expression.更多还原

【Abstract】 In this study,we investigated the effect of presence of hepatitis C virus(HCV) core upon the expression of IncRNAs,mRNAs,and circRNAs in human Huh7 hepatoma(Huh7) cells,and focused on the possible carcinogenic mechanism of HCV core protein.The data showed that 4851 lncRNAs,4785 mRNAs,and 823 circRNAs were 2.0-fold up-regulated in Huh7-core cells.The genes in the enriched set were associated with macromolecule metabolic processes and DNA damage response etc.By the Kyoto encyclopedia of genes and genome pathway analyses,the identified 10 genes from the selected 14 genes were higher or lower expression in Huh7-core cells than that of Huh7-vector cells analyzed by qPCR.Interestingly,overexpression of miR122 and miR204 partly abrogated the expression of TGFBRAP1 and HOTTIP,and increased the HPCAL1 expression in the predicted carcinogenic pathways.Our data suggested that the pathways of miR204-HPCAL1-lncRNAHOTTIP and miR122-TGFBRAP1 likely involved in the carcinogenic progress due to HCV core of presence,and that miR122 and miR204 overexpression might inhibit the progress by down-regulation of TGFBRAP1 and HOTTIP expression.更多还原