【作者】 李津乐； 王敏；

【Author】 Jinle Li;Min Wang;State Key Laboratory of Oral Diseases,Sichuan University,West China School of Stomatology;

【机构】 四川大学华西口腔医学院·口腔疾病研究国家重点实验室；

【摘要】 目的:原发性高血压(PH)可导致严重的全身病变,且其作为全身性的系统疾病,可引起机体免疫反应的异常。机体免疫失调也被认为是牙周病的主要病因,临床回顾性研究表明其与PH有着密切的关系。但PH如何对牙周病产生影响,并无实验研究其在作用机制。方法:我们通过建立PH牙周病小鼠模型,探讨了STAT3在Ang Ⅱ对TLR4调控过程中可能的作用方式。结果:我们的研究表明PH可通过血管紧张素Ⅱ(Ang Ⅱ)对Toll样受体4(TLR4)在牙周病变区域进行调控,导致持续的免疫反应。体外研究进一步证明,树突细胞(DCs)中信号传导与转录激活因子3(STAT3)在Ang Ⅱ对TLR4的调控过程中可能起着关键的作用。我们结果表明在牙周病伴发PH时,Ang Ⅱ可通过STAT3促进TLR4样受体在DCs中的表达增加,从而活化DCs使其持续参与免疫反应并导致牙周组织破坏。更多还原

【Abstract】 The consequence of primary hypertension(PH) is a serious disease that will cause severe damage of human body, which is also considered as a systematic disease that will cause abnormal of immune system. Periodontal diseases are also considered to have close relationship with dysregulation of immune response. Retrospective studies have shown that periodontal diseases are closely related to PH. However, there is still no experimental studies show that how PH affect the development of periodontal diseases. [Methods] we established the periodontitis accompanied by PH mouse model to explore the possible mechanism of STAT3 on how AngⅡ regulates expression and function of TLR4. [Results] Our preliminary studies demonstrated that PH may affect the periodontal diseases by Angiotensin(AngⅡ), which will regulate the expression and function of Toll-like receptor 4(TLR4) and the continued immune responses in the lesion areas. The in vitro study further showed that Signal transducer and activator of transcription 3(STAT3) may play a critical function during the process of regulation of AngⅡ on TLR4 in dendritic cells(DCs).Therefore, we hypothesize that AngⅡ may activate the STATs, which will then increase the function and expression of TLR4 by unclear mechanism in DCs. The increase of TLR4 will lead to uncontrolled immune response and the following activation of osteoclasts mediated bone destruction in periodontitis lesion areas. Our current result will make a great impact on the progress of how PH affect the oral infectious bone diseases.更多还原