【作者】 李刘泽木； 成仿云； 钟原； 文书生； 王新； 黄弄璋；

【Author】 LI Liu-zemu;CHENG Fang-yun;ZHONG Yuan;WEN Shu-sheng;WANG Xin;HUANG Nong-zhang;Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding,National Engineering Research Center for Floriculture,Beijing Laboratory of Urban and Rural Ecological Environment and College of Landscape Architecture,Beijing Forestry University;

【机构】 花卉种质创新与分子育种北京市重点实验室国家花卉工程技术研究中心城乡生态环境北京实验室园林学院北京林业大学；

【摘要】 本文以芍药与牡丹组间杂种（伊藤杂种）‘Bartzella’的鳞芽为外植体,初步建立了其微繁殖体系。主要结果包括:①‘Bartzella’的最佳取材时间为3月下旬;②WPM培养基中Ca（NO₃）₂浓度提高至原浓度的4倍(2224mg·L^-1),可显著促进组培苗的生长和增殖;添加有机物水解酪蛋白0.25g·L^-1也可在一定程度上提高组培苗增殖率。③生根培养前,将无根苗先接种于培养基1/2 MS中复壮培养10天,然后转入1/2 MS+腐胺1.0mg·L^-1+IBA 1.0mg·L^-1培养基中诱导根原基,冷处理12天后转常温黑暗环境下诱导培养30天,最后在根形成培养基1/2MS+活性炭4.0g·L^-1中培养20天,生根率可达75.51%。④生根质量对移栽成活率影响显著,根数多于3条且愈伤组织小的生根苗移栽成活率高达73.58%。更多还原

【Abstract】 The in-vitro cultured shoots of intersectional hybrids between herbaceous and tree peonies ’ Bartzella’ were used to develop a protocol for micro-propagation.The results showed that:①The optimal sampling time of ’ Bartzella’ was late March.②Modification of WPM basal medium on fourfold Ca（NO₃）₂ and adding 0.25 g · L^-1 casein acid hydrolysate were effective on the improving of multiplication and growth of ’ Bartzella’.③For optimal rooting,the shoots were cultured on 1/2MS for 10 days,then cultured on 1/2 MS + putrescine 1.0mg · L^-1 + IBA 1.0mg · L^-1 for 30 days to induce rooting after a cold treatment（4℃）for 12 days,and then transferred onto root formation medium(1/2MS + Activated carbon 4.0mg · L^-1)for20 days,so that 75.51%rooting rate was achieved finally.④The rooting quality had significantly effect on survival of in-vitro rooted plantlets in ’ Bartzella’.Plantlets with root number more than three and less callus survived 73.58%.更多还原