白藜芦醇对肾性高血压大鼠脑动脉瘤形成的抑制作用

【作者】 郭勇； 苏军华； 范琼英； 石桂芳； 华国祥；

【机构】 石家庄市第一医院医务处； 河北医科大学第二医院检验科； 石家庄市第一医院科研处； 冀中能源峰峰集团总医院； 邯郸陶瓷集团总公司医院；

【摘要】 目的观察白藜芦醇对肾性高血压大鼠脑动脉瘤形成的抑制作用及其对NF-κB活性的影响。方法首先制作大鼠脑动脉瘤模型,将其随机分为对照组、模型组和治疗组,应用免疫组化及EMSA检测NFkB的动态变化。结果模型组动脉瘤发生率约12%,治疗组仅发现部分动脉呈瘤前病变,无明显的动脉瘤产生。NF-κB在各组脑动脉壁中均有表达,对照组NF-κB活性较弱;模型组随着大鼠血压的增加成正相关性,术后1月在左右活性增至高峰,并延续至术后2月,之后逐渐下降,术后4月左右降至正常水平;治疗组NF-κB活性被显著抑制,随着血压的增加,NF-κB活性仅有轻度增加,无活性高峰出现。结论 NF-κB的高表达在脑动脉瘤形成的发病机制中起重要作用,白藜芦醇能够抑制NF-κB的高表达可能是防止动脉瘤的发生的机制之一。更多还原

【Abstract】 Objective to observe the resveratrol for renal hypertensive rat aneurysm formation inhibition and its effect on the activity of the NFkB.Method first of all make aneurysm model rats,which were randomly divided into control group,model group and treatment group,the application of immunohistochemical and EMSA detection NF-κBdynamic change.Results the incidence of aneurysm model group,about 1.2%,the treatment group found only part of the arterial lesion before a tumor,no obvious aneurysm.The NF-kB in each group were expressed in the brain artery walls,weak control the NF-κB activity;Model group are positively correlation with the increase of blood pressure of rats,increased to a peak in January after the activity,and last until February,after declining,postoperative April down to normal levels;Treatment group the NF-κB activity was significantly inhibited,with the increase of blood pressure of rats,the NF-κB activity only slightly increased,the activity of no obvious peak appeared.Conclusion high expression of the NF-κB in play an important role in the pathogenesis of cerebral aneurysm formation,high expression of resveratrol can inhibit the NF-κB may be prevent aneurysm of one of the mechanisms.更多还原