【作者】 杨小明； 方洋洋； 刘亚丽； 刘盼君；

【Author】 Yang Xiao-Ming;Fang Yang-Yang;Liu Ya-Li;Liu Pan-Jun;The School of Food and Biological Engineering,Jiangsu University;

【机构】 江苏大学食品与生物工程学院；

【摘要】 The interaction between Ginkgol C15:l and Herring Sperm DNA was studied by fluorescence spectra.The fluorescence spectra of the binding between Ginkgol C15:1 and AO-DNA was discussed at 20℃ and 37℃,and the results showed that the fluorescence quenching of Ginkgol C15:1 by AO-DNA was a static quenching procedure,meanwhile the binding constants,the number of binding sites and thermodynamic parameters were obtained.The main binding force was hydrogen bond force on the basis of thermodynamic parameters,which indicate the interaction between Ginkgol C15:1 and AO-DNA was intercalation.The results of ionic strength method,viscosity measurements and the thermal denaturation experiments further demonstrated that intercalative binding was the main mode between Ginkgol C15:1 and AO-DNA.更多还原

【Abstract】 The interaction between Ginkgol C15:l and Herring Sperm DNA was studied by fluorescence spectra.The fluorescence spectra of the binding between Ginkgol C15:1 and AO-DNA was discussed at 20℃ and 37℃,and the results showed that the fluorescence quenching of Ginkgol C15:1 by AO-DNA was a static quenching procedure,meanwhile the binding constants,the number of binding sites and thermodynamic parameters were obtained.The main binding force was hydrogen bond force on the basis of thermodynamic parameters,which indicate the interaction between Ginkgol C15:1 and AO-DNA was intercalation.The results of ionic strength method,viscosity measurements and the thermal denaturation experiments further demonstrated that intercalative binding was the main mode between Ginkgol C15:1 and AO-DNA.更多还原