【作者】 石蕊寒； Majid Hussain Soomro； 佘锐萍； 杨依霏； 吴桥兴； 胡凤姣； 李恒； 毛晶晶； 赵月； 杜芳； 王铜铜； 郝文卓； 苑雪瑞； 安俊卿；

【Author】 SHI Ruihan;Majid H S;SHE Ruiping;Yang Yifei;Wu Qiaoxing;Hu Fengjiao;Li Heng;Mao Jingjing;Zhao Yue;Du Fang;Wang Tongtong;Hao Wenzhuo;Yuan Xuerui;An Junqing;College of Veterinary Medicine,China Agricultural University;

【机构】 中国农业大学动物医学院；

【摘要】 目前关于戊型肝炎患者伴随发生神经功能障碍的报道日渐增多,为观察戊型肝炎病毒(Heptaitis E virus,HEV)对中枢神经系统的侵害作用,本研究用四型猪源HEV感染沙鼠,结合RT-n PCR,ELISA,组织病理学、超微结构病理学方法以及免疫组织化学技术,对于中枢神经系统中戊型肝炎病毒进行检测,并对其引发的病理变化进行观察分析。结果显示在感染后7到28天内沙鼠的脑和脊髓组织中都能检测到HEV的正链及负链RNA;光镜及电镜观察显示在HEV阳性的沙鼠脑和脊髓组织中出现了神经元变性坏死,小胶质细胞结节,淋巴细胞浸润和血管袖套等病理变化,并且血脑屏障相关的结构出现了损伤。以上结果表明HEV能够在脑和脊髓组织中进行复制并引发病理损伤。更多还原

【Abstract】 Recent literatures have been reporting cases of patients developing neurological manifestations involved with HEV infection. In order to determine the neurotropic feature of HEV, swine HEV was used to inoculate Mongolian gerbils. RT-n PCR, ELISA, histopathology, ultrastructural pathology and immunohistochemistry method were conducted to investigate the replication of HEV in the central nervous system and the consequent pathological changes. Both positive- and negative-strand HEV RNA was detectable in brain and spinal cord from 7 to 28 dpi(days post inoculation) and various pathological changes such as neuron necrosis, microglia nodule, lymphocyte infiltration, perivascular cuff and compromised blood-brain barrier(BBB) structure were observed. These results indicated that HEV is able to replicate in the central nervous system which hammers the causative role of HEV in the pathogenesis of neurologic disorders.更多还原