【作者】 丁霄； 李朋波； 潘转霞； 吴翠翠； 曹彩荣； 朱永红； 杨六六； 夏芝；

【Author】 Ding Xiao;Li Pengbo;Pan Zhuanxia;Wu Cuicui;Cao Cairong;Zhu Yonghong;Yang Liuliu;Xia Zhi;Shanxi Key Laboratory of Cotton Germplasm Resources Utilization and Molecular Design Breeding,Cotton Research Institute,Shanxi Academy of Agricultural Sciences;

【机构】 山西省农业科学院棉花研究所/棉花种质资源利用与分子设计育种山西省重点实验室；

【摘要】 4-香豆酸-辅酶A连接酶(4-coumarate:CoA ligase,4CL)处于苯丙烷代谢特异合成途径的分支点上,参与植物白藜芦醇、木质素及类黄酮的合成,在不同发育阶段表达受到启动子的调节。通过在线预测软件Plant CARE及CHOPCHOP对陆地棉Gh4CL启动子进行分析,启动子功能元件预测显示陆地棉Gh4CL1和Gh4CL2的启动子含有TATA-box和CAAT-box核心元件、MYB转录因子结合元件、光响应元件,乙烯响应元件、赤霉素响应元件,Gh4CL1特有的功能元件有醇溶蛋白顺式作用调控元件、水杨酸响应元件,Gh4CL2特有的功能元件有ARE元件、TA-rich region、茉莉酸甲脂响应元件;PAM序列分析结果显示,Gh4CL1启动子有53个PAM序列,Gh4CL2启动子有57个PAM序列,其中28个PAM序列相同。Gh4CL启动子的分析为今后利用CRISPRi定向调控陆地棉Gh4CL表达提供了候选编辑位点。更多还原

【Abstract】 4-coumarate:coenzyme A ligase(4CL)is located on the branch point of phenylpropanoid biosynthesis pathway which involved in the synthesis of resveratrol,flavonoids and lignin in plant.Its expression is regulated by the promoter at different stages of development.The results of analyzing Gossypium hirsutum Gh4 CLpromoters by online prediction software plant CARE and CHOPCHOP,showed that Gh4CL1 and Gh4CL2promoters contained some typical cis-elements,such as TATA box and CAAT box,and some cis-acting elements AC-II,AE-box,Box I,I-box,ERE,GARE-motif;and specific functional elements in Gh4CL1 promoter are O2-site,TCA-element,specific functional elements in Gh4CL2 promoter are ARE,TA-rich region,TGACG-motif.Analysis of PAM sequence showed that Gh4CL1,Gh4CL2 promoters have 53,57 PAM sequences,and 28 of PAM sequences are the same.The analysis of the Gh4 CLpromoters provides candidate editing sites for the future use of CRISPRi to regulate the expression of Gh4 CLin Gossypium hirsutum.更多还原