【作者】 聂英军； 傅超美； 李莹； 胡慧玲； 廖婉； 严鑫； 甘彦雄； 刘珂伶； 刘哿依；

【Author】 NIE Ying-jun;FU Chao-mei;LI Ying;HU Hui-ling;LIAO Wan;YAN Xin;GAN Yan-xiong;LIU Ge-yi;College of Pharmacy,Chengdu University of TCM,state key laboratory and breeding base of Chinese medicine resources system on research and development and utilization of provincial and ministry construction;

【机构】 成都中医药大学药学院,中药资源系统研究与开发利用省部共建国家重点实验室培育基地；

【摘要】 目的优化醋莪术挥发油提取及包合工艺。方法采用水蒸气蒸馏法提取醋莪术挥发油,浸泡时间、加水量、提取时间为主要影响因素,以挥发油得率为评价指标,进行正交试验设计,对挥发油提取工艺进行筛选;然后采用饱和水溶液法对挥发油进行包合,以β-环糊精与挥发油的加入比例、包合时间及包合温度为主要影响因素,以醋莪术挥发油包合率和包合物得率为评价指标,进行正交实验设计,对挥发油包合工艺进行筛选。采用人工神经网络法,对提取与包合的试验参数进一步进行优化。结果经正交试验与人工神经网络法优化的提取与包合工艺条件分别为:取醋莪术饮片100g,加入8倍量水,浸泡1h,水蒸汽蒸馏提取6h,即得挥发油:取挥发油1 ml,β-环糊精与挥发油加入比为8:1,包合温度50℃,包合1h,即得挥发油包合物。结论优化提取与包合工艺能显著提高原药材中挥发油利用率,该工艺稳定可行,重复性较好。更多还原

【Abstract】 Objective:To optimize the volatile oil extraction and clathration process of vinegar prepared rhizoma zedoariae.Method s:The volatile oil was extracted by steam distillation,orthogonal experiment was adopted to screen the methods of vinegar prepared rhizoma zedoariae volatile oil extraction process,by set immerse time,the volume of water,extraction time as the main influence factor,and use the essential oil yield as evaluation indicator to screen the volatile oil extraction methods;saturated water solution method was adopted to clathrate the volatile oil,by set the volume of β-cyclodextrin and volatile oil,clathration time,clathration temperature as main influence factor,use oil bearing ratio and inclusion ratio as evaluation indicator to screen the volatile oil clathration methods.Analysis method of artificial neural network was selected to optimize the figures on the result of extraction and clathration process.Result s:After the optimize of artificial neural network analysis,the suitable extraction and clathration prosess is:8 times water,immerse 1 h,extract6 h,acquire volatile oil.β-cyclodextrin:volatile oil=8:1,clathrate 1 h,clathration temperature50℃,acquire clathrate compound.Clathrate compound can be aquired.Conclusion:This optimized extraction and clathration process can promote the use ratio of volatile oil volume,the process is stable and repeatable.更多还原