【作者】 侯丽； 许晶； 赵林涛； 宋延平；

【Author】 HOU Li;XU Jing;ZHAO Lin Tao;ZHANG Ya Yue;SONG Yan Ping;Department of Oncology and Hematolog,Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine;Shaanxi University of Traditional Chinese Medicine;Shaanxi Institute of Traditional Chinese Medicine;

【机构】 北京中医药大学东直门医院肿瘤血液科； 陕西中医学院； 陕西省中医药研究院药理室；

【摘要】 目的观察新加良附方联合5-Fu对人胃癌细胞(SGC-7901)裸鼠移植瘤生长抑制作用及其对肿瘤组织中Fas及FasL蛋白表达的影响。方法:建立移植性人胃癌细胞(SGC-7901)裸鼠移植瘤模型,随机分为模型对照、5-FU组及新加良附方高、中、低剂量组、联合给药组(新加良附中剂量+5-FU组)6组。连续给药10d,计算肿瘤抑制率,检测Fas/FasL蛋白表达。结果联合给药组抑瘤率为65.28%,与模型对照组比较,有统计学意义(P<0.05);与5-Fu组和新加良附高剂量比较联合给药组抑瘤率升高明显,有统计学意义(P<0.05);联合给药组上调肿瘤组织Fas蛋白表达,下调FasL蛋白表达,与模型对照组比较,有统计学意义(P<0.05,P<0.01);与5-Fu组和新加良附高剂量组比较,联合给药组Fas蛋白表达升高明显(P<0.05),FasL蛋白表达下降明显(P<0.05)。结论联合给药组抑瘤率高于新加良附高剂量组及5-FU组,在调节FasL、Fas表达方面优于新加良附高剂量组及5-Fu组,显示了两药协同作用的优势。更多还原

【Abstract】 Objective:To observe the effect of Xinjialiangfu(XJLF) Formula on human gastric cancer(SGC-7901) tumor growth in nude mice and its impact on Fas and FasL expression in tumor tissue.Methods:The animal model of SGC-7901 xenografted nude mice was established,and all the mice were randomly divided into 6 groups;namely model control group,5-Fu group,XJLF Formula high-dose group,XJLF Formula medium-dose group,XJLF Formula low-dose group and drug combination group.After 10 days of continuous treatment,tumor inhibition rate was calculated and the expression of Fas and FasL was detected by immunohistochemistry techniques.Results:The tumor inhibition rate of drug combination group was 65.28%,which was significantly increased compared to the model control group(P<0.05),5-FU group(P<0.05) and XJLF high-dose group(P<0.05),respectively.Meanwhile,the Fas expression was significantly up-regulated and the FasL expression was significantly down-regulated in drug combination group,compared to the model control group(P<0.05),P<0.01),5-FU group(P<0.05),(P<0.05) and XJLF Formula high-dose group(P<0.05),(P<0.05),respectively.Conclusion:The tumor inhibition rate of drug combination group is higher than XJLF Formula high-dose group and 5-FU group.Also combination group has stronger effect compared to model control group in terms of down-regulation of Fas expression and up-regulation of FasL expression,which indicates a benefit of XJLF and 5-Fu combination treatment.更多还原