【作者】 冯中红； 杨成德； 薛莉； 陈秀蓉；

【Author】 FENG Zhong-hong;YANG Cheng-de;XUE Li;CHENG Xiu-rong;College of Grassland,Gansu Agricultural University;Key Laboratory of Grassland Ecosystem(Gansu Agricultural University),Ministry of Education;Pratacultural Engineering Laboratory of Gansu Province;Sino-U.S.Center for Grazingland Ecosystem Sustainability;

【机构】 甘肃农业大学草业学院 草业生态系统教育部重点实验室 甘肃省草业工程实验室 中-美草地畜牧业可持续发展研究中心；

【摘要】 采用含菌平板抑菌圈测定法,从高寒草地牧草内生细菌中筛选到一株对番茄丁香假单孢叶斑病（Pseudomonas syringa pv.tomato）拮抗能力较强的菌株262XY2’,其抑菌圈直径为1.22cm。经Salkowski比色法和钼锑抗比色法测定,该菌株具有产IAA和溶磷能力,在无氮培养基上可以生长,具有固氮能力。革兰氏染色为阳性,菌体杆状,大小为（0.50×1.03）^0.57×1.82μm。根据形态学特征,结合16S rDNA和gyrB基因序列相似性分析,将菌株262XY2’鉴定为枯草芽孢杆菌Bacillus subtilis。通过室内盆栽防效试验,结果表明该菌株对番茄丁香假单孢叶斑病的防效达68.71%。该研究结果为262XY2’的进一步开发利用提供了依据。更多还原

【Abstract】 An antagonistic strain 262XY2’ of endophytic bacteria from alpine meadow pasture was isolated and screened by detection of inhibition zone of bacterial plate,which had strong antagonistic ability to Pseudomonas syringa pv.tomato,and the diameter of inhibition zone was 1.22 cm.The strain had the abilities of nitrogen fixation and IAA secretion by using the Salkowski colorimetry and Mo-Sb colorimetry,it also could grow on the culture without nitrogen medium.It was Rod-shaped with size of（0.50×1.03） ^0.57×1.82 μm,Gram-positive.The strain was identified as Bacillus subtilis according to its morphology and homology analysis of the 16 S rDNA and gyrB gene sequence.The results showed that the control effect on the bacterial spot of tomato leaves was up to 68.71%.So this result provided basis for strain 262XY2’ to make further development and utilization.更多还原