【作者】 郝蓉蓉； 杨成德；

【Author】 HAO Rong-rong;YANG Cheng-de;College of Grassland,Gansu Agricultural University;Key Laboratory of Grassland Ecosystem(Gansu Agricultural University),Ministry of Education;Pratacultural Engineering Laboratory of Gansu Province;Sino-U.S.Center for Grazingland Ecosystem Sustainability;

【机构】 甘肃农业大学草业学院草业生态系统教育部重点实验室,甘肃省草业工程实验室,中-美草地畜牧业可持续发展研究中心；

【摘要】 马铃薯作为一种重要的粮饲兼用作物,病害多发成为影响其产业发展的重要因素。本试验通过对峙培养,从实验室保存的185株土壤细菌和内生细菌中筛选出对马铃薯干腐病菌(F.solani)具有良好拮抗效果的细菌30株,抑菌率均在70%以上,且对马铃薯丝核病菌(Rhizoctonia solani Ktihn)也有较好的拮抗效果,抑菌率可达60%以上。对拮抗细菌进行溶磷、固氮、产吲哚乙酸功能的测定,结果表明7株菌有溶磷功能,其中265ZY1、263XY1、262XY1溶磷功能较好,分别为40μg/ml、38.94μg/ml,42μg/ml;19株菌有产吲哚乙酸功能,265ZY3、265XY6产吲哚乙酸功能较好,分别为110μg/ml,147μg/ml;17株菌有固氮功能。对这30株细菌的亲和性测验结果表明265ZY1、263XY1、262XY1、265ZY3、265XY6等五株细菌可两两亲和,根据16srDNA基因序列分析结果,将其分别鉴定为Bacillus vallismortis,Bacillus vallismortis,Bacillus amyloliquefaciens,Bacillus amyloliquefaciens,Bacillus methylotrophicus。更多还原

【Abstract】 Potato is an important crop and controlling its multiple diseases is a crucial factor in its industrial development.In this project,soil bacteria were screened to identify possible antagonistic bacteria to control Fusarium solani,a causative agent of the devastating disease.Thirty out of 185 soil and endophytic bacteria had a 70% antagonistic effect towards F..solani.Furthermore,these bacteria also had a 60-65%antagonistic effect against Rhizoctonia,another potato pathogen(Rhizoctonia solani K(u|¨)hn).The antagonistic bacteria are able to dissolve phosphorus,absorb NO3,and are able to produce indole acetic acid as determined by a functional assay.Seven species have dissolved phosphorus function,and the ones that function better were 265ZY1,263XY1,262XY1(phosphate solubilization was 40μg / ml,38.94μg /ml,42μg / ml respectively).Nineteen species produced indole acetic acid;species 265ZY3,265XY6 produce indole acetic acid function better(110μg/ml,147μg / ml respectively).Seventeen species have nitrogen-fixing capabilities.Five out of 30 of these bacteria(265ZY1,263XY1,262XY1,265ZY3,265XY6) are able to grow with any other bacteria.Twoamphiphilic and bacteria,according to 16 srDNA gene sequence analysis,they were identified as Bacillus vallismortis,Bacillus vallismortis,Bacillus amyloliquefaciens,Bacillus amyloliquefaciens,Bacillus methylotrophwus.更多还原