【作者】 番华彩； 杨佩文； 郭志祥； 白亭亭； 曾莉；

【Author】 Fan Hua-cai;Yang Pei-wen;Guo Zhi-xiang;Bai Ting-ting;Zeng Li;Institute of Agriculture Environment and Resource,Yunnan Academy of Agricultural Sciences;

【机构】 云南省农业科学院农业环境资源研究所；

【摘要】 由多主棒孢菌[Corynespora cassiicola（Berk&Curt）Wei]引起的香蕉棒孢霉叶斑病是危害香蕉生产的一类主要叶部病害,本研究对分离自云南香蕉种植区的香蕉棒孢霉叶斑病病原菌进行了生物学特性研究及室内药剂筛选。生物学特性测定结果表明:病原菌菌丝生长最适培养基为PDA;最适碳、氮源分别为乳糖、甘露醇和蛋白胨;最适温度为28³0℃;最适pH为7.0;在玉米粉培养基上病菌产孢量最高;光照与黑暗交替有利于产孢;菌丝致死温度为58℃,15 min,分生孢子的致死温度是51℃,5 min。采用菌丝生长速率法测定7种杀菌剂对该病原菌的毒力大小,结果表明:丙环唑、多抗霉素、苯甲·丙环唑效果较好,EC₅₀值分别为4.2470、4.7223、5.9986ug/mL。更多还原

【Abstract】 The banana leaf spot disease caused by Corynespora cassiicola is one of the most banana leaf disease,The biological characteristics and indoor screening of fungicides on C.cassiicola of banana in Yunnan were studied.Biological characteristics measurement showed that PDA was the optimal medium for hyphal growth.The optimum carbon and nitrogen sources were lactose,mannitol and peptone;The optimal temperature was 28℃-30℃;the optimal pH was 7.0;Corn meal medium was the optimal culture medium for mycelium growth;Alternating light and darkness could promote sporulation;Mycelium and conidia lethal temperature were 58 ℃ for 15 min and 51 ℃for 5 min,respectively.Toxicity test of 7 fungicides to C.cassiicola was cariied out in the laboratory by using mycelium growth rate method,and the results showed that the best fungicides were propiconazole,multi antimycin and benzoic Propiconazole.The EC₅₀ values were 4.2470 ug/mL、 4.7223 ug/mL、 5.9986ug/mL,respectively.更多还原