【作者】 陈杰； 熊焕明；

【Author】 Jie Chen;Huan-Ming Xiong;Department of Chemistry, Fudan University;

【机构】 复旦大学化学系；

【摘要】 采用两步共聚的方法合成了荧光可调的水溶性Zn O@polymer核壳结构量子点,可以负载抗癌药物DOX形成Zn O@polymer-DOX纳米复合物。Zn O@polymer-DOX水溶液的紫外吸收光谱及荧光光谱表现出了Zn O与DOX两者的混合特征。通过控制酸度能从Zn O@polymer-DOX表面释放DOX,但在这种酸度下不会破坏DOX的稳定性。适当浓度的Zn O@polymer纳米粒子在48h培养后对U251细胞没有明显的细胞毒性,但相同浓度的Zn O@polymer-DOX可以有效的杀死癌细胞,杀伤率甚至超过了DOX本身。所有实验结果表明了载药机制:首先Zn O@polymer-DOX纳米粒子内吞进入细胞,然后被溶酶体分解释放出DOX,后者进入细胞核杀死癌细胞。更多还原

【Abstract】 We developed a two-step copolymerization method for the synthesis of core–shell-structured Zn O@polymer QDs with stable luminescence in aqueous solutions. The obtained Zn O@polymer can load DOX to form a Zn O@polymer–DOX nanocomposite. After adding Zn O@polymer QDs to an aqueous solution of DOX, the UV/Vis absorption spectra and the photoluminescence(PL) spectra show the mixed features of both Zn O@polymer and DOX. The DOX molecules have been released from the surfaces by controlling the p H,while DOX is stable at this p H. The Zn O@-polymer nanoparticles have no visible cytotoxic effect on U251 cells after 48 h treatment, but shows high cytotoxicity after DOX loading. All results suggest that the drug delivery mechanism includes the cellular internalization of Zn O@polymer–DOX, followed by its biodegradation in endosomes/lysosomes, and the subsequent penetration of the drug into the nucleus.更多还原