【作者】 王延平； 李娜； 肖迎红； 杨小弟；

【Author】 Yanping Wang;Na Li;Yinghong Xiao;Xiaodi Yang;Jiangsu Provincial Key Laboratory of novel power cell,College of Chemistry and Materials Science,Nanjing Normal University;

【机构】 江苏省新型动力电池重点实验室,南京师范大学化学与材料科学学院；

【摘要】 <正>石墨烯作为新兴的纳米碳材料,其特殊的二维结构,使其除具有纳米效应外,还具有完美的量子隧道效应,内部电子运动速率可达光速的1/300[1],将石墨烯修饰在电极表面可促进电子转移[2],这些性质为它在电化学适体传感器中的应用提供了可能。更多还原

【Abstract】 A novel impedimetric aptasensor was constructed for thrombin determination based on graphene fonctionalized electrode(RGO/GCE) obtained by reducing graphene oxide on the surface of glassy carbon electrode(GO/GCE). Subsequently, a 5’ amine-modified aptamer sequence was immobilized onto RGO/GCE to detect thrombin of different concentrations. Changes in the interfacial electron transfer resistance(Ret) of the aptasensor were monitored using the redox couple of an [Fe(CN)6]3- /[Fe(CN)6]4- probe. This work demonstrates that graphene is a promising nanomaterial in the design of electrochemical aptasensor and opens the possibility of a real application to diagnostics or medication investigation of protein.更多还原