【作者】 张卫卫； 符贵红； 王元； 湛嘉； 房文红i； 沈锦玉； 周俊芳； 姚嘉赟；

【Author】 ZHANG Wei-wei;FU Gui-hong;WANG Yuan;ZHAN Jia;FANG Wen-hong;SHEN Jin-yu;ZHOU Jun-fang;YAO Jia-yun;Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation and utilization, Ministry of Agriculture,East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences;(2.Ningbo Academy of Science and Technology for Inspection and Quarantine;(3.Zhejiang Institute of Freshwater Fisheries;

【机构】 中国水产科学研究院东海水产研究所农业部东海与远洋渔业资源开发利用重点实验室； 宁波市检验检疫科学技术研究院； 浙江省淡水水产研究所；

【摘要】 本文采用UPLC-MS/MS法,研究了阿维菌素泼洒用药后在模拟水产养殖池塘生态系统中的行为,探明了阿维菌素在水体中的消解规律,在底泥、伊乐藻(Elodea nuttallii)和鱼蟹水产动物体内的蓄积与消除规律。结果显示,以6μg/L剂量单次泼洒用药后,水体中阿维菌素消解较快,其半衰期为63.8 h。阿维菌素在养殖水环境中消减的同时,逐渐由水体向底泥、伊乐藻和水产动物迁移。底泥中阿维菌素峰浓度、AUC和半衰期分别为1.25μg/kg、469.2μg/kg·h和115.5 h,伊乐藻中的相应值分别为8.75μg/kg、2521.7μg/kg·h和315.0 h,伊乐藻对阿维菌素有明显的吸收和富集作用。该模拟系统中的异育银鲫(Carassius auratus gibelio)对阿维菌素具有明显的吸收,其血液、肾脏、鳃、肝脏和肌肉组织阿维菌素的最高浓度(Cmax)依次为50.9、45.37、21.25、15.47和11.9μg/kg;而该系统中的中华绒螯蟹(Eriocheir sinensis)仅鳃组织检出阿维菌素,其Cmax在12 h为8.08μg/kg,血淋巴、肌肉和肝胰腺等组织均未检出阿维菌素。生物富集系数FBC值显示对阿维菌素的富集浓度由高到低依次为鲫鱼>伊乐藻>中华绒螯蟹>底泥,显示阿维菌素在不同分配相和不同生物组织的富集作用差异大。更多还原

【Abstract】 The UPLC-MS/MS method were used to study the avermectin(AVM) behavior in a simulatedaquaculture pond by a single medication.The degradation of AVM in water, as well as the accumulation and elimination of AVM in sediments, elodea nuttallii and the tissues of fish and crab were explored. The results showed that when AVM was splashedto the aquatic environment and existedin the water at the concentration of 6 μg/L, AVM in water dissipated fast and the half-life time was 63.8h. At rhe same time AVMkept transferring to the sediments,elodea nuttalliiand aquatic animals. The AVM peak concentration, AUC and half-life in the sediments were 1.25 μg/kg, 469.2 μg/kg·h and 115.5 h, respectively. The corresponding values in theelodea nuttalliiwere 8.75 μg/kg, 2521.7 μg/kg·hand 315.0 h, respectively.These results indicated that AVM was absorbed and enriched in the elodea nuttallii. In this simulated system, crucian carp(Carassiusauratusqibelio)could obviously absorb and enrich AVM from water, and the peak concentration in the blood, kidney, gill, liver and muscle,were 50.9, 45.37, 21.25, 15.47 and 11.9 μg/kg, respectively. While in Chinese mitten crab(Eriocheirsinensis) AVM only could be detected in the gill with the peak concentration of 8.08 μg/kg, but it couldn‘t be detected in the haemolymph, muscle and hepatopancreas. The bioconcentration factor FBCin descending order was crucian carp>elodea nuttallii> Chinese mitten crab> sediments.The results proved that there were different for absorption and enrichment in different environment components and in different tissues in the same aquatic animal.更多还原