【作者】 苏发文； 高鹏程； 么宗利； 周凯； 王慧； 仲启铖； 来琦芳；

【Author】 SU fawen;GAO pengcheng;YAO zongli;ZHOU kai;WANG hui;ZHONG qicheng;LAI qifang;East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences;

【机构】 盐碱地渔业工程技术研究中心东海水产研究所中国水产科学研究院； 上海海洋大学水产与生命学院；

【摘要】 以盐碱水对虾养殖池塘优势微藻铜绿微囊藻(Microcystis aeruginosa)、小球藻(Chlorella sp.)为研究对象,采用正交试验,研究了不同温度(20℃、25℃、30℃)和光照强度(2000lx、4000lx、6000lx)组合下两种微藻生长过程中对养殖水环境p H的影响。结果显示,处于对数生长期的铜绿微囊藻和小球藻均能使水环境p H值上升,且p H的变化因温度和光照强度的交互作用而差异性显著(P<0.05)。在本试验范围内,不同温度和光照强度组合条件下,铜绿微囊藻的生长均能使水环境p H上升至9.0以上,温度25℃和光照强度2000lx条件下,水环境p H达到峰值10.84,藻密度达最大值1.1×107 cells/ml;小球藻生长促使水环境p H的上升,并随温度升高、光照强度增强而增加,结果均维持在7.62±0.094,温度30℃和光照强度6000lx条件下,水环境p H达到峰值7.73,藻密度达最大值8.0×106 cells/ml。本研究结果表明,铜绿微囊藻生长对水环境p H的影响显著高于小球藻,其作用结果已超过养殖生物适宜生长p H范围,本结果可为下一步讨论不同类群浮游植物CO2-CO32--HCO3-利用途径做基础,同时为盐碱水养殖池塘水质调控提供理论指导。更多还原

【Abstract】 Microcystis aeruginosa and Chlorella sp.as two dominance species of microalgae in Saline-alkali aquaculture ponds,was culture at different combinations of temperature(20℃,25℃ and 30℃) and light intensity(2000lx,4000 lx and 6000lx) in order to study the effects of the growth of M. aeruginosa and Chlorella sp.on the p H value by Orthogonal experimental. The results showed that that temperature, light intensity and their interactions had significant effects on p H of algae medium, and the p H increased with the exponential phase growth of M.aeruginosa and Chlorella sp.A higher p H value of M.aeruginosa medium was observed at 2000 lx and 25 ℃.Under such conditions, p H of M.aeruginosa medium could reach to the maximum value of 10.84 and the maximum algal density could reach to 1.1×107 cells/ml. The p H of Chlorella sp.medium increased with the increase in temperature and light intensity. A higher p H value of M.aeruginosa medium was observed at 6000 lx and 30 ℃. The maximum value of p H and algal density could reach to 7.73 and 8.0×106 cells/ml respectively.The results of this study showed that M.aeruginosa have a significant effect on the p H value higher than that of Chlorella sp.,the result can provide theoretical guidance for Saline-alkali aquaculture.更多还原