【作者】 黄珺； 黄洪辉； 吴风霞； 戴明； 齐占会；

【Author】 Jun Huang;Honghui Huang;Fengxia Wu;Ming Dai;Zhanhui Qi;Key Laboratory of South China Sea Fishery Resources Development and Utilization,Ministry of Agriculture;Key Laboratory of Marine Fishery Ecology Environment of Guangdong Province;South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences;College of Fisheries and Life Science of Shanghai Ocean University;

【机构】 农业部南海渔业资源开发利用重点实验室广东省渔业生态环境重点实验室中国水产科学研究院南海水产研究所； 上海海洋大学水产与生命学院；

【摘要】 为研究嗜盐杆菌溶藻菌株(Halobacillus sp.HSQAY1)产生的溶藻活性物质的特性,实验通过改变p H、温度、反复冻融条件研究其对中肋骨条藻溶藻活性的影响,并采取超滤、乙醇沉淀法等手段对溶藻物质进行了分离纯化研究。结果显示,该菌株产生胞外溶藻物质在酸性条件下(p H=3)丧失溶藻活性,而在p H 5~11时仍保持较高溶藻活性(溶藻效率87.0%~98.8%);随温度的升高溶藻活性虽略有下降趋势,但110℃高温处理后溶藻率仍可达83.2%;反复冻融对其溶藻活性没有影响。超滤结果表明该溶藻物质分子量>10 k Da;乙醇浸提相没有溶藻活性,沉淀相溶藻率为75.1%。更多还原

【Abstract】 The characteristics of algicidal substance produced by Halobacillus sp.(HSQAY1) under different p H, heat treatment, and repeated freeze-thaw cycles were investigated. The results showed that the algicidal substance was not significantly affected by alkali and effectively inhibited the growth of Skeletonema costatum, but lost the algicidal activity under stresses with acid treatment. In the heat treatment and the repeated freeze-thaw cycles treatment, the algicidal activity of the algicidal substance did not change significantly. The molecular weight of algicidal substance was > 10 k Da. Interestingly, observation of ethanol precipitation demonstrated that the ethanol precipitation has significantly algicidal activity. however, the water-layer did not inhibit the growth of the Skeletonema costatum.更多还原