【作者】 韦小瑜； 李世军； 游旅； 田克诚； 唐光鹏； 王定明；

【Author】 WEI Xiao-yu;LI Shi-jun;YOU Lv;TIAN Ke-cheng;TANG Guang-peng;WANG Ding-ming;Research Institute of Infectious Disease Control and Prevention,Guizhou Provincial Center for Disease Control and Prevention;

【机构】 贵州省疾病预防控制中心；

【摘要】 目的对来自贵州省的8例空肠弯曲菌病疑似病例进行病原学诊断与分子流行病学特征分析,为病例的确诊和贵州省空肠弯曲菌病疫情的预防和控制提供科学依据。方法对8例疑似病例粪便和血液标本进行细菌分离,采用传统方法和多重PCR方法对空肠弯曲菌可疑菌株进行鉴定,应用脉冲场凝胶电泳分型技术(PFGE)对空场弯曲菌分离株进行分析。结果8例空肠弯曲菌病疑似病例标本均分离出空肠弯曲菌可疑菌株,PCR扩增出空肠弯曲菌预期大小的目的基因片段,PFGE显示8株菌经SamI酶切后产生7~10条DNA条带,聚类分析显示,8株菌株分为7个PFGE带型,8株菌的相似性>50%,其中GZ201004和GZ201005两株腹泻病例来源分离株相似性为100%,血培养血液标本分离株GZ201201与粪便标本分离株GZ201007相似性为66.7%。结论8例空肠弯曲菌病疑似病例均检出空肠弯曲菌,PFGE显示GZ201004和GZ201005两株为同一来源,但8例病例来源菌株存在PFGE带型多态性,该研究结果为病例的确诊和疫情的预防和控制提供了科学依据。1更多还原

【Abstract】 Eight patients with suspected cases of C.jejuni were etiologically diagnose and analyze in this study to provide scientific basis for the confirmation of the cases of human campylobacteriosis in Guizhou Province.Blood or feces of 8 suspected patients were employed to isolate bacteria strains.Conventional and multi-PCR techniques were applied to identify suspicious bacteria strains.The C.jejuni strains were analyzed by using Pulsed Field Gel Electrophoresis(PFGE).Suspicious strains of C.jejuni were isolated from all the 8 suspected patients of campylobacteriosis and anticipated genes fragment were detected with multi-PCR.With the digestion of restriction enzyme SmaI,the 8 C.jejuni strains were divided into 7 PFGE patterns with 7-10 DNA bands.Cluster analysis showed that the gross similarity of 8strains of C.jejuni was more man 50%.The similarity of PFGE patterns between strain GZ201004 and GZ201005 from diarrhea patients was as high as 100%,while the similarity of strain GZ201201 and GZ201007 was 66.7%.Moreover,C.jejuni were detected from all the suspected patients of campylobacteriosis.PFGE results indicated that strains GZ201004 and GZ201005 was from the same source,while all the 8 isolates showed PFGE polymorphism.更多还原