【作者】 叶川； 何志旭； 尚显文； 刘日光； 李江伟； 向阳； 周玉坤； 范建楠； 宁旭； 杨华； 吴程； 庄勇；

【机构】 贵阳医学院附属医院骨科；

【摘要】 Bio-electrospraying(BES) is a technique for directly jetting living cells that has significant implications for tissue engineering and regenerative medicine.The effect of BES on human adipose-derived stem cells(hASCs),which are a promising cell source for cell-based therapy and tissue engineering,remains unknown.Therefore,we examined hASC viability,proliferation and multilineage differentiation capacity after BES.An hASC suspension was successfully electrosprayed through continuous,stable and linearly directed electrospray at the voltage of 10 kV and flow rate of 3 mL/h.Morphological observations,trypan blue and CCK-8 assays revealed that lOkV electrosprayed cells were viable and proliferated at a rate similar to that of the controls(0 kV).However,at 20 kV,BES became unstable,and cell viability was reduced.Moreover,histological staining,immunocytochemical staining,real-time RT-PCR and western blots indicated that hASCs electrosprayed at 10 kV retained their multilineage potential and can successfully differentiate into chondrogenic,osteogenic and neurogenic lineages.These results demonstrate that BES does not significantly affect cell morphology,viability or multipotency and that BES can be a safe technology for hASCs delivery in tissue engineering and advanced cellular therapeutic applications.更多还原

【Abstract】 Bio-electrospraying(BES) is a technique for directly jetting living cells that has significant implications for tissue engineering and regenerative medicine.The effect of BES on human adipose-derived stem cells(hASCs),which are a promising cell source for cell-based therapy and tissue engineering,remains unknown.Therefore,we examined hASC viability,proliferation and multilineage differentiation capacity after BES.An hASC suspension was successfully electrosprayed through continuous,stable and linearly directed electrospray at the voltage of 10 kV and flow rate of 3 mL/h.Morphological observations,trypan blue and CCK-8 assays revealed that lOkV electrosprayed cells were viable and proliferated at a rate similar to that of the controls(0 kV).However,at 20 kV,BES became unstable,and cell viability was reduced.Moreover,histological staining,immunocytochemical staining,real-time RT-PCR and western blots indicated that hASCs electrosprayed at 10 kV retained their multilineage potential and can successfully differentiate into chondrogenic,osteogenic and neurogenic lineages.These results demonstrate that BES does not significantly affect cell morphology,viability or multipotency and that BES can be a safe technology for hASCs delivery in tissue engineering and advanced cellular therapeutic applications.更多还原