【作者】 王涛； 陆胜民； 夏其乐；

【Author】 Tao Wang;Shengmin Lu;Qile Xia;Key Laboratory of Fruits and Vegetables Postharvest and Processing of Zhejiang Province,Institute of Food Science,Zhejiang Academy of Agricultural Science;Zhejiang Normal University;

【机构】 浙江省农业科学院食品科学研究所浙江省果蔬保鲜与加工技术研究重点实验室； 浙江师范大学化学与生命科学学院；

【摘要】 为了充分利用杨梅疏果。研究了大孔树脂柱层析法从杨梅疏果核仁粗提液中分离纯化苦杏仁苷的工艺。通过大孔树脂的静态吸附试验,筛选得到D101型为最合适的大孔树脂,其对杨梅疏果核仁中苦杏仁苷的吸附过程符合为二级动力学模型:通过大孔树脂动态吸附试验。确定D101型树脂对杨梅疏果核仁中苦杏仁苷的分离过程符合Langmuir和Freundlich模型.是一种基于吸附能量均匀分布的单分子层吸附;通过动态吸附解吸试验,得到D101型大孔树脂制备分离苦杏仁苷的最佳纯化参数为:上样液中苦杏仁苷浓度为129-358 mg/L,上样流速2 BV/h;先用3 BV的纯净水以2 BV/h的流速冲柱,随后用11 BV的乙醇-水溶液(40:60,v/v)进行洗脱,流速为2 BV/h。经过一次D101型大孔吸附树脂处理,产物中苦杏仁苷的含量从4.83%增加到82.00%,约增加了17倍,其回收率为77.94%。更多还原

【Abstract】 To utilize the low-value thinned bayberry(Myrica rubra Sieb.et Zucc.) kernels(TBKs)waste,an efficient method using macroporous adsorption resins(MARs) for separation and purification of amygdalin from TBKs crude extracts was developed.An aqueous crude sample was prepared from a methanol TBK extract,followed by resin separation.A series of MARs were initially screened for adsorption/desorption of amygdalin in the extract,and DlOl was selected for characterization and method development.The static adsorption data of amygdalin on DlOl was best fitted to the pseudo-second-order kinetics model.The solute affinity towards DlOl at 30 ℃ was described and the equilibrium experimental data were well-fitted to Langmuir and Freundlich isotherms.Through one cycle of dynamic adsorption/desorption,the purity of amygdalin in the extract,determined by HPLC,increased about 17-fold from 4.8%to 82.0%,with77.9%recovery.The results suggested that DlOl resin effectively separate amygdalin from TBKs.更多还原