【作者】 李玫璐； 谭量量； 裘卫； 尹志红； 沈立荣；

【Author】 Li Meilu;Tan Liangliang;Qiu Wei;Yin Zhihong;Shen Lirong;College of Biosystems Engineering and Food Science,Fuli Institute of Food Science,Zhejiang Key Laboratory for Agro-Food Processing,Zhejiang University;Hangzhou Biyutian Health Food Co.,Ltd;

【机构】 浙江大学生物系统工程与食品科学学院馥莉食品科学研究院浙江省农产品与食品加工重点实验室； 杭州碧于天保健品有限公司；

【摘要】 近年来国际上研究证明,王浆主蛋白(MRJPs)是蜂王浆的主要的生物活性成分,但目前尚缺乏环保而高效的产业化分离技术,为此,我们开展了MRJPs的超滤分离优化工艺研究。首先就水料比、pH、离子强度三种单因素对MRJPs提取率的影响进行单因素分析,然后通过正交试验对MRJPs的超滤提取工艺进行优化,最后对MRJPs分离产物冻干粉和滤后液进行成分测定。结果表明:最佳提取工艺为水料比5:1 mL/g,pH 7.0,离子强度0.5 mol/L。在最佳工艺条件下,MRJPs的提取率为82.63%,冻干粉含可溶性蛋白91.00%、水分0.01%、总糖15.22%、王浆酸2.00%,超滤副产物—滤后液中王浆酸和总糖的回收率分别为3.60%和2.11%。超滤工艺具有无溶剂污染、王浆成分可回收利用,适合规模化生产的优点,为MRJPs分离提取提供了新的工艺方法,为王浆深加工和MRJPs蛋白产品开发提供了科学依据。更多还原

【Abstract】 It has been confirmed that the Major royal proteins(MRJPs) are the main biological compositions in royal jelly(RJ).However,the industry isolation technique of MRJPs,which is beneficial for environmental protection with higher high-efficency is still lacked.Therefore,we implemented the research isolating MRJPs from RJ with ultrafiltration separation.Firstly,three factors,pH,ionic strength and water ratio effected extraction rate of MRJPs were investigated,respectively.Then,the orthogonal experiments of ultrafiltration separation,and the composition of the lyophilized powder of MRJPs and the by-products were analyzed,respectively.The results showed that optimal condition is as bellows,water ratio 5:1,ionic strength 0.5 mol/L and pH 7.0.Under the optimal condition,82.63%of MRJPs was extracted from fresh RJ.It was determined that the lyophilized powder of MRJPs contained 91.00%soluble protein,0.01%moisture,2.00%10-HDA and 15.22%total sugar.Besides,the by-products contained 10-HDA and total sugar were recycled,the recycling rate of the two components reached 3.60%and 2.11%,respectively.This method with many advantages,such as no solvent pollution and fully utilization of 10-HDA has provided a new technology for MRJPs separation by first using ultrafiltration in deep process of RJ,and will be hopeful for large scale application in RJ process industry.更多还原