【作者】 段小洁； 符天明； Charles M.Lieber；

【Author】 Xiaojie Duan;Tian_Ming Fu;Charles M.Lieber;Department of Biomedical Engineering,College of Engineering,Peking University;Department of Chemistry and Chemical Biology,Harvard University;School of Engineering and Applied Sciences,Harvard University;

【机构】 北京大学工学院生物医学工程系； Department of Chemistry and Chemical Biology,Harvard University； School of Engineering and Applied Sciences,Harvard University；

【摘要】 对生物电信号,如神经信号的检测是生命科学和生物医学工程领域的重要研究内容。因为空间分辨率和生物相容性的要求,纳米级的检测电极一直是人们追求的目标,但常规检测中信号随电极尺寸的减小大大减弱,给这一目标的实现带来挑战。本工作通过在生物电信号检测中应用场效应晶体管的检测原理,并在电极结构设计中引入纳米管/纳米线的三维异质或同质结构,成功发展了多种亚100 nm甚至亚10 nm的细胞内和细胞外电极,实验测量和理论计算均证明纳米电极的尺寸即使降低到与离子通道蛋白尺寸相当的5 nm,电极仍有足够的时间分辨率和带宽来检测千赫兹的生物电信号。结合磷脂双层膜化学修饰对电极/细胞界面的调控,实现了活细胞内动作电位和静息电位的高信噪比、高时间空间分辨率、低干扰检测,以及同一细胞内和细胞网络内等不同尺度下的高密度多位点平行检测。这些电极的进一步发展和应用将为大面积、高分辨生物电信号检测提供强有力的工具。更多还原

【Abstract】 The detection of bioelectric signals, such as neural signals, is crucial for both fundamental biological studies and biomedical applications. Because of their advantage in biocompatibility and spatial resolution, nanosize detection electrodes have been of great interest for researchers. However, in conventional recording, the signals are significantly attenuated along with the decrease of electrode size, which makes it difficult to get effective nanosize electrodes. By applying new detection scheme and designing three-dimensional nanowire/nanotube hetero- or homo-structures, this work developed sub-100 nm and even sub-10 nm intracellular and extracellular detection electrodes. With the chemical modification of electrodes which enables an effective control over electrode/cell interface, the applicant realized the detection of action potential and resting potential with minimal invasiveness, high signal-to-noise ratio, and high spatio-temporal resolution. By developing multiple individually addressed nano-electrodes, the applicant also achieved the simultaneous, multi-site recording of action potentials with high-density from both single cells and cell networks.更多还原