【作者】 赵思奇； 郝旖； 唐玉海；

【Author】 Siqi Zhao;Yi Hao;Yuhai Tang;Institute of Analytical Science, School of Science, Xi’an Jiaotong University;College of Pharmacy, University of Xi’an Jiaotong;

【机构】 西安交通大学理学院； 西安交通大学药学院；

【摘要】 3-丙烯酰胺基苯硼酸(AAPBA)是一种硼酸衍生物,自身独特的结构及良好的亲水性和生物相容性使得其非常适用于生物大分子的印迹。本工作以牛血清白蛋白(BSA)为模板分子,以带双键的磁性纳米粒子为载体,以AAPBA为功能单体,制备了核-壳结构的磁性分子印迹纳米粒子(Fe3O4@BSA-MIPs)。这种新的印迹方法通过BSA与AAPBA之间的可逆共价作用在Fe3O4表面形成了特异性识别位点。通过吸附性能考察,所得聚合物不仅可以将BSA同其它蛋白质分离,还可以在实际牛血样中对BSA选择性富集。更多还原

【Abstract】 3-acrylamidophenylboronic acid(AAPBA), as one of the boronic acid derivates possessing distinctive structure and the attractive properties of hydrophilicity and biocompatibility, is suitable for biomolecule imprinting. In this work, the core-shell molecularly imprinted magnetic nanoparticles(Fe3O4@BSA-MIPs) were synthesized using bovine serum albumin(BSA) as the template, vinyle functionalized magnetic nanoparticles as supporters, and AAPBA as the functional monomer. The novel imprinting strategy using reversible covalent interaction of BSA was described for creating BSA-specific recognition cavities on 3-acrylamidophenylboronic acid-immobilized Fe3O4 nanoparticles. The results of binding experiments showed that the Fe3O4@BSA-MIPs could not only separation the template from other proteins, but also enrich BSA from real bovine blood sample.更多还原