【作者】 周敏； 陈剑； 韦万兴； 刘盛；

【Author】 ZHOU Min,CHEN Jian,WEI Wan-xing*,LIU Sheng School of Chemistry and Chemical Engineering,Guangxi University,Nanning 530004,China

【机构】 广西大学化学化工学院；

【摘要】 采用高速逆流色谱(HSCCC)法对珠子草粗提物中的三种木脂素niranthin(1)、hypophyllanthin(2)和phyllanthin(3)进行分离。考察了温度和转速对溶剂体系1(正己烷:乙酸乙酯:甲醇:水=3:2:3:2,V/V/V/V)和溶剂体系2(正己烷:乙酸乙酯:乙醇:水=6:4:5.5:5,V/V/V/V)的分离效果的影响,结果表明,在温度25.0℃、转速800r/min、流动相流速2.0mL/min的条件下,三种化合物均能够有效地分离。利用此条件下的溶剂体系1,进样量达到257.3mg的粗提物可以有效分离得到niranthin84.8mg(纯度69.97%)、hypophyllanthin31.9mg(纯度90.24%)、phyllanthin68.2mg(纯度86.78%)。更多还原

【Abstract】 The effects of temperature and rotational speed on isolation of three lignans niranthin(1),hypophyllanthin(2),and phyllanthin(3) from phyllanthus niruri Linn by high-speed counter-current chromatography were investigated,under the selected solvent system 1(n-hexane:ethyl acetate:methanol:water=3:2:3:2,V /V /V /V) and solvent system 2(n-hexane:ethyl acetate:ethanol:water=6:4:5.5:5,V /V /V /V).The results showed that at flow-rate of 2.0 mL /min for the mobile phase at 25.0℃,and clockwise rotation of 800 r /min,the three compounds were all effectively separated.In addition,under the optimized conditions of solvent system 1,a larger sample size of crude extract(257.3 mg) was also effectively separated to give niranthin(84.8 mg),hypophyllanthin(31.9 mg),and phyllanthin(68.2 mg) with 69.97%,90.24%,86.78% purity,respectively.更多还原