【作者】 胡国柱； 漆琼瑶； 高幼奇； 文珠；

【Author】 Hu Guozhu,Qi Qiongyao~1,Gao Youqi,Wen Zhu~(2#) (The People’s Hospital of Jiangxi Province,Nanchang 330006;1 Grade 2009 master graduate student,Nanchang university medical college,Nanchang 330006;2 Medical Science Institute of Jiangxi,Nanchang 330006)

【机构】 江西省人民医院神经内科； 南昌大学医学院； 江西省医学科学研究所；

【摘要】 目的:探讨西洋参皂苷(PQLS)抑制缺0诱导的胚鼠大脑皮层神经细胞凋亡的作用机理。方法:实验分组:正常对照组、凋亡阳性组、西洋参皂苷0.025g/L组、0.05g/L组、0.1g/L组、0.2g/L组;SD胚大鼠原代大脑皮层神经细胞体外无血清缺氧-复氧培养;应用MTT、DNA电泳、免疫细胞化学染色等方法观察西洋参皂苷的适宜剂量、DNA凋亡条带及凋亡调节蛋白Bcl-2、Bax、Caspase-3的表达率。结果:①缺氧条件下PQLS在0.05～0.1 g/L促进缺氧的神经细胞增殖较凋亡阳性组提高31.17%～37.65%(P<0.01)。②0.05～0.2g/L的PQLS使缺氧的神经细胞DNA只出现3条凋亡条带(凋亡阳性组6条带),且条带浓度也明显降低。③PQLS在0.025g/L～0.1g/L显著减少缺氧的神经细胞表达Bax(67.02±1.69%～57.97±2.6%,凋亡阳性组72.19±3.97%,P<0.05～0.01)和Caspase-3(62.27±2.02%～50.34±2.65%,凋亡阳性组68.62±1.40%,P<0.05～0.01):提高缺氧的神经细胞表达Bcl-2(44.22±3.73%～63.76±2.96%,凋亡阳性组35.61±2.16%,P<0.01)和Bcl-2/Bax比值(0.69±0.11～1.1 1±0.04,凋亡阳性组0.56±0.1 1,P<0.05～0.01)。结论:PQLS通过降低缺氧的神经细胞表达Bax、Caspase-3,提高Bcl-2的表达及Bcl-2/Bax比例阻断缺氧诱导的神经细胞凋亡,保护缺氧的神经细胞。更多还原

【Abstract】 Objective:To explore the mechanisms of Panax quinquefolium L.saponin(PQLS) suppressing apoptosis of embryonic rat cerebral cortical neurons induced by anoxia.Methods:The Experiments were divided into normal control,apoptosis positive group,PQLS 0.025g/L group,PQLS 0.05g/L group,PQLS 0.1 g/L group,PQLS 0.2g/L group.Primary anoxia-reoxygenation culture of SD embryonic rat cerebral cortical neurons was performed in serum-free in vitro.The appropriate dosage of PQLS,apoptosis strip of fragmented DNA,and expression rates of anti-apoptotic protein Bcl-2 and apoptosis proteins Bax and Caspase-3 were determined by MTT assay,DNA electrophoresis and immunocytochemistry.Results:①With increasing concentration in PQLS 0.05g/L～0.1g/L the proliferation rates of anoxic neurons still increased gradually comparing with apoptosis positive group (31.17%～37.65%,P<0.01).②In PQLS 0.05g/L～0.2g/L there were three strips of fragmented DNA of anoxic neurons to be watched,which was less than in apoptosis positive group not only in numbers(six strips) but also in volume.③With increasing concentration in PQLS 0.025g/L～0.1g/L the anoxic neurons expressed Bax would decrease gradually(67.02±1.69%～57.97±2.6%) comparing with it in apoptosis positive group(72.19±3.97%,P<0.05～0.01),the anoxic neurons expressed Caspase-3 would decrease gradually(62.27±2.02%～50.34±2.65%) comparing with it in apoptosis positive group(68.62±1.40%,P<0.05～0.01),the anoxic neurons expressed Bcl-2 would increase gradually (44.22±3.73%～63.76±2.96%),comparing with it in apoptosis positive group(35.61±2.16%, P<0.01),and the Bcl-2/Bax ratio would increase gradually(0.69±0.11～1.11±0.04) comparing with it in apoptosis positive group(0.56±0.11.P<0.05～0.01).Conclusion:By means of reducing the anoxic neurons expressed Bax and Caspase-3,and increasing the anoxic neurons expressed Bcl-2 and ratio of anoxic neurons expressed Bcl-2 and anoxic neurons expressed Bax the Panax quinquefolium L. saponin prevents apoptosis of embryonic rat cerebral cortical neurons caused by anoxia and protects neurons caused by anoxia.更多还原