Sonchus asper extract inhibits LPS-induced oxidative stress andpro-inflammatory cytokine production in RAW264.7 macrophages

【作者】 王兰；

【机构】 Colloge of Life Science ,Henan Normal University；

【摘要】 Sonchus asper is an herb that is used extensively to treat bronchitis, asthma, wounds,burns, and cough. To determine its mechanism of action, we examined the effects of anethyl acetate fraction(EAF) of S. asper on nitric oxide(NO) production and prostaglandin(PG)-E2 levels in lipopolysaccharide(LPS)-stimulated RAW264.7 macrophages. We observedsignificant inhibition associated with suppression of oxidative stress as evidencedby increased endogenous superoxide dismutase(SOD) activity and intracellular glutathione(GSH) levels, decreased reactive oxygen species(ROS) generation and lipid peroxidation,and restoration of the mitochondrial membrane potential. To confirm its antiinflammatoryeffects, expression of inducible NO synthase(iNOS), cyclooxygenase-2(COX-2), tumor necrosis factor(TNF)-α, and the anti-inflammatory cytokines IL-1β and IL-6 wereanalyzed by semi-quantitative RT-PCR. EAF treatment significantly reduced the expressionof all of these factors in a dose-dependent manner, and enhanced expression of the antioxidantsMnSOD and heme oxygenase-1(HO-1). Additionally, HPLC fingerprint resultssuggest that rutin, caffeic acid, and quercetin may be the active ingredients in EAF. Takentogether, these findings imply that the anti-inflammatory effect of EAF on LPS-stimulatedRAW264.7 cells is mediated by suppression of oxidative stress.更多还原

【Abstract】 Sonchus asper is an herb that is used extensively to treat bronchitis, asthma, wounds,burns, and cough. To determine its mechanism of action, we examined the effects of anethyl acetate fraction(EAF) of S. asper on nitric oxide(NO) production and prostaglandin(PG)-E2 levels in lipopolysaccharide(LPS)-stimulated RAW264.7 macrophages. We observedsignificant inhibition associated with suppression of oxidative stress as evidencedby increased endogenous superoxide dismutase(SOD) activity and intracellular glutathione(GSH) levels, decreased reactive oxygen species(ROS) generation and lipid peroxidation,and restoration of the mitochondrial membrane potential. To confirm its antiinflammatoryeffects, expression of inducible NO synthase(iNOS), cyclooxygenase-2(COX-2), tumor necrosis factor(TNF)-α, and the anti-inflammatory cytokines IL-1β and IL-6 wereanalyzed by semi-quantitative RT-PCR. EAF treatment significantly reduced the expressionof all of these factors in a dose-dependent manner, and enhanced expression of the antioxidantsMnSOD and heme oxygenase-1(HO-1). Additionally, HPLC fingerprint resultssuggest that rutin, caffeic acid, and quercetin may be the active ingredients in EAF. Takentogether, these findings imply that the anti-inflammatory effect of EAF on LPS-stimulatedRAW264.7 cells is mediated by suppression of oxidative stress.更多还原