【作者】 张然； 林爱红； 沈振华；

【Author】 ZHANGRan,CHEN Gui-bing,LINAI-hong,etal.(Shenzhen Center for Disease Control and Prevention,Shenzhen 518055,Guangdong)

【机构】 深圳市疾病预防控制中心；

【摘要】 目的了解深圳市环境中不同水体军团菌污染和血清型别分布状况。方法利用传统的检验方法与实时荧光PCR技术相结合,对深圳市环境中243份水源进行军团菌的检测,用传统细菌分离培养法分离鉴定,用实时荧光PCR法加以验证。结果共检测环境水源243份,52份水样检出军团菌阳性,其中49份水样为嗜肺军团菌,3份水样为非嗜肺军团菌,军团菌和嗜肺军团菌总检出率分别为21.4%(52/243)和20.2%(49/243);空调系统嗜肺军团菌检出率为25%(29/116),宾馆淋浴热水嗜肺军团菌检出率为23.3%(20/86),二次供水、游泳池水、景观水池水均未检出军团菌。血清型以LP1和LP6为优势菌型,占检出菌的65.3%(32/49)和22.5%(11/49),LP3、LP5、LP6、LP7、LP10均检出1株。结论深圳市环境水源中军团菌污染主要分布于空调冷却塔水和宾馆淋浴热水中,嗜肺军团菌血清型以LP1、LP6为主,相关部门需引起足够重视,防止军团菌病发生。更多还原

【Abstract】 [Objective]To investigate the distribution state of Legionellae contamination and serological typing Legionella in environmental water systems of Shenzhen[Methods]243 samples of environmental water were detected,isolated and cultured with traditional method.Real time fluorescent PCR method was used to confirm the results.[Results]In 243 samples of environmental water,The Legionella was detected in 52 samples of environment water,including 49 samples of Legionella pneumophila,3 samples of non- Legionella pneumophila,the contamination rate were 21.4%(52 / 243 )和20.2%(49 / 243).The Legionella pneumophila rate of cooling tower water, shower warm water were25%(29 / 116),23.3%(20 / 86),other were 0.00%[Couclusiou]The results suggest that Legionellae distributes in cooling tower water and shower warm water at hotels in Shenzhen.it’s a potential threat to public health.更多还原