【作者】 莫浩联； 司徒潮满； 方良； 陈润莉； 牛姬飞； 李丽廉； 周海涛；

【Author】 Mo Haolian,Situ Chaoman,Fang Liang,et al.Shenzhen Futian Center for Disease Control and Prevention(Shenzhen 518040,China)

【机构】 深圳市福田区疾病预防控制中心；

【摘要】 目的建立可控性强的病毒富集方法基本条件,科学评价空气微生物采样器的采样效率,为消毒监测和传染病监控提供依据。方法通过气溶胶发生器模拟空气中流感病毒,使用约30μm孔径超细静电纤维膜、0.2μm孔径Teflon静电膜和AGI—30大气采样器对病毒进行富集监测,随后运用荧光定量PCR,分析探讨不同采样器的不同采样条件对病毒的富集效能。结果在一定的采样条件下(膜表面<450Pa),使用超细静电纤维膜和Teflon膜对空气中流感病毒气溶胶的阻留吸附率98.0%以上,收集率也较高,而AGI—30则较低。结论AGI-30不宜用于长时间和较高流速采样,而较大孔径和小孔径的静电膜都具较高的富集效能,而较大孔径静电膜更便宜易得和更易于推广。更多还原

【Abstract】 Objective To establish the stronger controllable basic conditions for virus enrichment methods,scientific evaluate the sampling efficiency of microbial air sampler, provide evidence for sterilization surveillance and infectious disease monitoring.Method Through the aerosol generator simulating influenza virus in the air,monitor the virus concentrate using aperture approximately 30μm superfine static electricity fiber membrane, the aperture 0.2μm Teflon static electricity membrane and the AGI-30 atmosphere sampling instrument,and then using fluorescence quota PCR to analyze and discuss the efficiency.Result When membrane surface<450Pa,using superfine static electricity fiber membrane and Teflon static electricity membrane,the filtration and collection rate is higher then the AGI-30.Conclusion AGI-30 is not suitable for using in the long time and high flow rate sampling,but the static electricity membrane of big and small aperture has higher concentrate potency,and the latter is much cheaper and easier in the promotion.更多还原