脾虚大鼠有机阴离子转运肽oatp2a1基因表达及意义探讨

【作者】 郝尧坤； 林佑武； 张诗军； 陈泽雄； 潘爱珍； 董霄； 胡斌；

【机构】 中山大学附属第一医院中医科； 中山大学达安基因诊断中心；

【摘要】 目的:通过观察脾虚状下大鼠oatp2a1基因表达来探讨oatp2a1在湿浊转运中的作用。方法:32只大鼠随机分为4组,分别为正常组(6只)、正常+AA大鼠模型组(6只)、脾虚大鼠模型组(10只)、脾虚+AA大鼠模型组(10只)。采用皮下注射利血平的方法造脾虚大鼠模型,造模成功后给予相应组别大鼠马兜铃酸灌胃3天,最后采用实时荧光定量PCR方法检测各组大鼠肺、肝、肾、胃、小肠、大肠等组织中oatp2a1的基因表达情况。结果:oatp2a1在上述6种脏器中都有不同程度的表达。肝组织中,脾虚组大鼠模型oatp2a1的表达量较正常组明显升高(P=0.035,P<0.05);小肠组织中,脾虚组大鼠模型中oatp2a1的表达较正常组明显下降(P=0.004,P<0.01)。在给予马兜铃酸刺激下,小肠组织中,正常+AA组大鼠模型oatp2a1的表达较正常组明显下降(P=0.032,P<0.05)。结论:oatp2a1可能是机体参与湿浊运化的物质基础之一,在脾虚状态下大鼠小肠、肝组织中oatp2a1的表达变化提示小肠、肝等脏器在脾虚状态下的湿浊转运中可能发挥重要作用,脾主运化湿浊的功能,可能不仅包括胃肠道的功能在内,而且也可能包括一部分肝脏的功能,值得进一步研究。更多还原

【Abstract】 Objective:To explore mechanism of transportation and transformation of dampness by the way of the expression of Organic anion transporting polypeptide(OATP) superfamily member 2a1(OATP2al) mRNA in rat with spleen deficiency syndrome and the significance in transportation and transformation of dampness.Methods:32 SD rats were divided randomly into four groups:blank control group(6),blank control +AA group(6),spleen deficiency group(10),Spleen deficiency + AA group(10).After Reserpine-induced spleen deficiency model was made,intragastric administration of aristolochic acid(AA) was adopted for three days,the expression of oatp2al mRNA were detected in the tissues of lung,liver,kidney,stomach,small intestinal and large intestine in four groups by using Fluorescent Quantitative- Polymerase Chain Reaction(FQ-PCR).Results:The expression of oatp2al mRNA in above six tissues could be found.The expression of oatp2al mRNA in liver tissue of rat with spleen deficiency syndrome was up-regulated compared to blank control group(P=0.035,P<0.05),the expression of oatp2al mRNA in small intestinal tissue of rat with spleen deficiency syndrome was down-regulated compared to blank control group(P=0.004,P<0.01),the expression of oatp2al in intestinal tissue in blank control +AA group is down-regulated compared to blank control group(P=0.032,P<0.05).Conclusion:Oatp2al might be one of the material basis involved in transportation and transformation of dampness.The changes of expression of oatp2al mRNA in Small intestine,liver tissue suggests that small intestine,liver might play an important role in the transportation and transformation of dampness in the state of spleen deficiency.We further concluded that the function of spleen’s governing transportation and transformation of dampness was not only including the functions of the gastrointestinal,but also part of the liver function in some degree,which need to be further studied.更多还原