【作者】 刘泽发； 唐炎英； 李玲； 孙小武；

【Author】 LIU Zefa~(1,2),TANG Yanying~1,LI Ling~1 SUN Xiaowu~1 (1.Landiscape and Horticultural Institute of Hu nan Agriculture University,Changsha Hunan 410128,China;3.Hunan XiangYan seed co.,LTD.,Changsha,Hunan,410000;2.Hunan University of Humanities,Science and Technology,Loudi,Hunan,417000,China)

【机构】 湖南农业大学园艺园林学院； 湖南人文科技学院；

【摘要】 本文应用相关序列扩增多态性(Sequence-related Amplified Polymorphism,SRAP)标记和限制性位点扩增多态性(Restriction Site Amplification Polymorphism,RSAP)标记对印度南瓜品种杂交种子纯度进行了鉴定试验,结果表明筛选出的引物ElM5和R1R7可以很好的区分出红栗二号、红栗、锦栗二号、锦栗南瓜,利用RSAP分子标记R1R7可以检测出锦栗杂交种子纯度,分子标记检测结果与田间检测结果吻合率达到90%。更多还原

【Abstract】 SRAP,the sequence-related amplified polymorphism marker and RSAP,the restriction site amplification polymorphism marker were applicated,E1M5 and R1R7 can distinguish well Hong-Li No.2,Hong-Li,Jin-Li No.2 and Jin-Li pumpkin.R1R7 marker was used to test Jin-Li hybrid seed purity successfully,the marker test results and the field test results consistent rate of 90%.更多还原