【作者】 王真； 陆建良；

【Author】 WANG Zhen;LU Jianliang;Zhejiang University Tea Research Institute,866 Yuhangtang Road;

【机构】 浙江大学茶叶研究所；

【摘要】 茶树(Camellia sinensis)是多年生木本植物,拥有一个很大的基因组。抑制消减杂交技术(SSH)、cDNA末端快速扩增技术(RACE)、实时定量PCR技术(qRT-PCR)等基因工程技术的发展,为茶树表达序列标签(EST)的筛选、基因组测序提供了一种快速、经济高效、可靠的方法,使茶树SSH文库及cDNA文库的建立逐步完善。根据近几年国内茶树分子生物学的研究成果,本文总结了与茶树多酚类代谢、咖啡碱代谢、茶氨酸代谢、萜烯类代谢、抗逆性和一些基础代谢相关的基因的克隆及表达研究现状。更多还原

【Abstract】 Tea plant(Camellia sinensis) is one of the perennial woody plants and it has a large genome. The developments of suppression subtractive hybridization(SSH) technology, rapid amplification of cDNA ends(RACE) technology, quantitative real-time PCR(qRT-PCR) technology etc. have provided a kind of rapid, cost-effective method for filtering the expressed sequence tags(EST), genome sequencing of tea plant. The buildings of SSH library and cDNA library are gradually improved. According to the research achievements of tea plant molecular biology in recent years, this article summarizes the research status of some important functional genes which are connect with the metabolic pathways of tea polyphenols, theanine, terpenes etc.更多还原