【作者】 袁淑芳； 张丽娟； 郑嵘炅； 闫桂霞； 孙丽华； 张跃新；

【Author】 Shufang Yuan,Lijuan Zhang,Rongjiong Zheng,Guixia Yan,Lihua Sun,Yuexin Zhang Department of Infectious Diseases,First Affiliated Hospital of Xinjiang Medical University,Xinjiang,830011,China

【机构】 新疆医科大学第一附属医院感染病中心；

【摘要】 目的:观察慢性乙型肝炎、重型乙型肝炎和肝硬化患者肝组织中核转录因子NF-κB(nuclear transcription factor-κB)、肿瘤坏死因子-α(tumor necrosis factor-α)的表达与肝细胞损伤、肝纤维化的关系。方法:采用原位杂交技术、免疫组织化学方法检测10例非肝病对照者、66例病毒性肝炎患者肝组织中NF-κB、TNF-α蛋白的表达。结果:(1)病情越重,病毒性肝炎肝组织中NF-κB、TNF-α的表达水平越高。慢性乙肝(轻、中)、重型乙肝、肝硬化患者肝组织中NF-κB蛋白的阳性表达率分别为31.81%、73.33%、100%、100%,明显高于正常对照组10%(P<0.01);TNF-α蛋白的阳性表达率分别为50.0%、93.33%、100%、100%,明显高于正常对照组20%(P<0.01)。(2)随着慢性肝炎病理损伤的程度即肝组织炎症活动度(G)、肝纤维化程度(S)的增高,NF-κB、TNF-α蛋白表达增加,NF-κB、TNF-α的表达与炎症活动度、肝纤维化程度均具有等级相关关系(P<0.01)。(3)病毒性肝炎肝组织中NF-κB、TNF-α蛋白的表达呈正相关(rs=0.455,P<0.01)。结论:NF-κB、TNF-α参与了病毒性肝炎的病理损伤及肝纤维化的发病机制;二者的检测有助于判断肝细胞受损和肝纤维化程度。更多还原

【Abstract】 Objective:To explore the expressive state of NF-κB,TNF-αprotein in liver tissues of hepatitis B patients and its relationship with liver inflammation(G) and fibrosis(S).Methods: Sixty-six paraffin setions from patients with viral infection were studied and 10 cases of normal were as control.In situ hybridization was used to measure the expression of NF-κB protein in liver tissue.And Immunohistochemistry was employed to determine the expression of TNF-αprotein. Results:(1)The expression of NF-κB,TNF-αprotein in patients with viral hepatitis were increased with the worse of chronic liver injury.The positive rate of NF-κB protein in Chronic hepatitis B (mild,moderate),severe hepatitis B patients,Cirrhosis was 31.81%,73.33%,100%,100% respectively;significantly higher than the control group 10%(P <0.01);The positive rate of TNF-αprotein in Chronic hepatitis B(mild,moderate),severe hepatitis B patients,Cirrhosis was 50.0%, 93.33%,100%,100%respectively;significantly higher than the control group 20%(P <0.01);(2) With chronic hepatitis severity of pathological injury and liver fibrosis,the expression of NF-κB、TNF-αprotein gradually increased,the expression of NF-κB、TNF-αprotein were positive correlation with pathological injury and liver fibrosis(P <0.01).(3) There was were positive correlation between the expression of NF-κB and TNF-αprotein in liver tissues of hepatitis B patients(r_s =0.455,P<0.01).Conclusion:NF-κB,TNF-αinvolved in the pathological mechanisms of injury viral hepatitis and liver fibrosis;NF-κB,TNF-αmay reflect active hepatitis and liver tissue damage.Combined detection of NF-κB,TNF-αcan reflect degree of hepatocyte damage and liver fibrosis.更多还原