【作者】 吉云鹏； 徐志伟； 敖海清； 胡海燕； 陈攀；

【Author】 Ji Yunpeng~1 Xu Zhiwei~1 Ao Haiqing~1 Chen Pan~2 Hu Haiyan~1 1.Guangzhou University of Chinese Medicine Guangzhou 2.Guangxi University of Chinese Medicine Nanning

【机构】 广州中医药大学； Guangxi University of Chinese Medicine Nanning；

【摘要】 目的探讨四逆散对慢性应激大鼠空间学习记忆能力的影响。方法 SD大鼠随机分成正常对照组、应激模型组、四逆散低剂量组和四逆散高剂量组,每组10只。建立慢性心理应激动物模型,四逆散低剂量组和四逆散高剂量组分别给予四逆散高低不同浓度药液灌胃。采用Morris水迷宫实验检测大鼠空间学习记忆能力。结果定位航行实验结果示,各组逃避潜伏期组间存在显著差异(P<0.01)。与正常对照组比较,应激模型组、四逆散低剂量组和四逆散高剂量组潜伏期明显延长(P<0.01,P<0.05);与应激模型组比较,四逆散低剂量组潜伏期明显缩短(.P<0.05)。空间探索实验结果示,入水后前20s,与正常对照组相比,应激模型组及四逆散低剂量组大鼠穿越原平台区域次数明显减少(P<0.05),四逆散高剂量组无明显变化(P>0.05);与四逆散高剂量组相比,应激模型组和四逆散低剂量组明显减少(P<0.01,P<0.05)。入水后120s各组穿越原平台区域次数高低次序没有变化,仅四逆散高剂量组较四逆散低剂量组穿越原平台区域次数明显增加(P<0.05),其余各组均差异均无统计学意义(P>0.05)。结论 (1)长期慢性应激刺激可引起大鼠空间学习记忆能力损伤,导致空间学习记忆障碍。(2)疏肝代表方药四逆散可减轻慢性应激对大鼠空间学习记忆能力的损伤,提高慢性应激大鼠空间学习和记忆能力。(3)不同剂量的四逆散对慢性应激大鼠空间学习和记忆能力的保护作用也有变化。更多还原

【Abstract】 Objective To observe the effects of Sini Powder on spatial Learning and memory abilities of chronic stress rats.Methods SD rats were randomly divided into the normal group,the chronic stress group,the low dosage group and the high dosage group,10 rats in each group.The rats models of chronic stress were created and rats in the low dosage group and the high dosage group were given respectively with the low and high concentration of Sini Powder decoction.The learning and memory abilities of rats were examined by Morris Water Maze.Results In the place navigation test,there was the statistical significance among groups(P<0.01).Compared with the normal group,escape latency of rats were prolonged obviously in the chronic stress group,the low dosage group and the high dosage group (P<0.01,P<0.05).The escape latency of rats in the low dosage group was shorter than normal group (P<0.05).In the first 20 seconds of the spatial probe test,compared with the normal group,platform crossing times(times of crossing the former site of removed hidden platform) was significantly reduced in the chronic stress group,the low dosage group,and no obvious change in the high dosage group. Compared with the high dosage group,platform crossing times was significantly reduced in the chronic stress group and the low dosage group(P<0.01,P<0.05).In the 120 seconds of the spatial probe test,the order of platform crossing times was same as it did before.The platform crossing times of the high dosage group was more than that of the low dosage group.Beyond that,there was no obvious difference among groups(P>0.05).Conclusion①Chronic stress impairs spatial learning and memory abilities of stress rats.②Sini Powder could significantly alleviate the spatial learning and memory disturbance,improve spatial learning and memory abilities of chronic stress rats.③Different dosage of Sini Powder have different protective effect of spatial learning and memory abilities of chronic stress rats.更多还原