RNAi-mediated silencing of transcription factor SoxE gene expression in the silkworm ovary-derived BmN4 cells inhibits cell proliferation

【作者】 魏玲； Zhiqing Li； Daojun Cheng； Takahiro Kusakabe； Minhui Pan； Zhonghuai Xiang； 鲁成；

【Author】 Ling Wei~(1,2),Zhiqing Li~3,Daojun Cheng~1,Takahiro Kusakabe~3,Minhui Pan~1, Zhonghuai Xiang~1,Cheng Lu~(1*). 1.State Key Laboratory of Silkworm Genome Biology,Southwest University,Chongqing,China 2.School of Life Science,Southwest University,Chongqing,China 3.Laboratory of Silkworm Science,Kyushu University Graduate School of Biorcsource and

【机构】 State Key Laboratory of Silkworm Genome Biology,Southwest Univcrsity； School of Life Science,Southwest University； Laboratory of Silkworm Science,Kyushu University Graduate School of Biorcsource and； State Key Laboratory of Silkworm Genome Biology,Southwest University；

【摘要】 <正>Transcription factor SoxE is mainly expressed in gonad and involved in the regulation of gonad development and sex determination in animals.We based on silkworm ovary-derived cell line(BmN4-SID1) to survey the roles of BmSoxE on cell proliferation and predict the potential targets for BmSoxE.RNAi-mediated silencing of BmSoxE expression in BmN4-SIDI cells led to a decreased cell proliferation,which is likely resulted from a cell cycle arrest at the G1 phase after BmSoxE RNAi.A further genome-wide microarray screening revealed a significant expression change of a set of genes after BmSoxE RNAi.More than half of these genes contain conserved binding motifs for HMG-box of Sox protein and were expected as the targets for BmSoxE.Several sets of BmSoxE target candidates are associated with cell proliferation and show gonad-specific expression.These data will provide fundamental knowledge for investigating further the roles of BmSoxE in the control of cell cycle and silkworm development.更多还原

【Abstract】 Transcription factor SoxE is mainly expressed in gonad and involved in the regulation of gonad development and sex determination in animals.We based on silkworm ovary-derived cell line(BmN4-SID1) to survey the roles of BmSoxE on cell proliferation and predict the potential targets for BmSoxE.RNAi-mediated silencing of BmSoxE expression in BmN4-SIDI cells led to a decreased cell proliferation,which is likely resulted from a cell cycle arrest at the G1 phase after BmSoxE RNAi.A further genome-wide microarray screening revealed a significant expression change of a set of genes after BmSoxE RNAi.More than half of these genes contain conserved binding motifs for HMG-box of Sox protein and were expected as the targets for BmSoxE.Several sets of BmSoxE target candidates are associated with cell proliferation and show gonad-specific expression.These data will provide fundamental knowledge for investigating further the roles of BmSoxE in the control of cell cycle and silkworm development.更多还原