Cloning and characterization of the caspase family member BmDronc from embryonic cells of the silkworm,Bombyx mori

【作者】 张金叶； Wei Xu； Chun Pan； Hua-Shan Yi； Yan-Fen Hu； Juan Song； 潘敏慧； 鲁成；

【Author】 Jin-Ye Zhang~1,Wei Xu~2,Chun Pan~1,Hua-Shan Yi~1,Yan-Fen Hu~1,Juan Song~1,Min-Hui Pan~(1*),Cheng Lu~(1*) 1.State Key Laboratory of Silkworm Genome Biology,Institute of Sericulture and Systems Biology,Southwest University,Chongqing 400715,China 2.Key Laboratory of Biorheological Science and Technology,Ministry of Education,Bioengineering College, Chongqing University,Chongqing 400030,China

【机构】 State Key Laboratory of Silkworm Genome Biology,Institute of Sericulture and Systems Biology,Southwest University； Key Laboratory of Biorheological Science and Technology,Ministry of Education,Bioengineering College,Chongqing University；

【摘要】 <正>Caspases are a family of cysteinyl aspartate proteinases known for their essential roles in the regulation of apoptosis.To date,about 20 caspases have been identified although,few have been cloned and characterized in Bombyx mori.Two caspase-9 variants from the B.mori embryonic cell line(BmE),designated BmDroncL and BmDroncS were cloned.BmDroncL has three classic domains:a caspase recruitment domain(CARD) and large/small subunits,and is a homologue of Drosophila Dronc,human caspase-9,Aedes Dronc and fish caspase-9,while BmDroncS lacks the large subunit.Instantaneous expression of BmDroncL caused typical apoptosis in Sf-9 cells. Moreover,BmDronc gene expression increased markedly during the silkworm metamorphosis at the larva-pupa and pupa-moth stages,which suggested that BmDronc plays a crucial role during B. mori metamorphosis.更多还原

【Abstract】 Caspases are a family of cysteinyl aspartate proteinases known for their essential roles in the regulation of apoptosis.To date,about 20 caspases have been identified although,few have been cloned and characterized in Bombyx mori.Two caspase-9 variants from the B.mori embryonic cell line(BmE),designated BmDroncL and BmDroncS were cloned.BmDroncL has three classic domains:a caspase recruitment domain(CARD) and large/small subunits,and is a homologue of Drosophila Dronc,human caspase-9,Aedes Dronc and fish caspase-9,while BmDroncS lacks the large subunit.Instantaneous expression of BmDroncL caused typical apoptosis in Sf-9 cells. Moreover,BmDronc gene expression increased markedly during the silkworm metamorphosis at the larva-pupa and pupa-moth stages,which suggested that BmDronc plays a crucial role during B. mori metamorphosis.更多还原