【作者】 丛向明； 牛丽； 白喜云； 武彩红； 孙耀贵； 贺俊平； 白元生； 李宏全；

【Author】 CONG Xiang-ming~1,NIU Li~1,BAI Xi-yun~1,WU Cai-hong~2,SUN Yao-gui~1,HE Jun-ping~1,BAI Yuan-sheng~3,LI Hong-quan~1 (1.College of Animal Science and Veterinary Medicine,Shanxi Agricultural University,Taigu 030801,Shanxi;2.Jiangsu Animal Husbandry and Veterinary College,Taizhou 225300,Jiangsu;3.Shanxi Province Station of Ecological Animal Husbandry Industry Management,Taiyuan 030001,Shanxi)

【机构】 山西农业大学动物科技学院； 江苏畜牧兽医职业技术学院； 山西省生态畜牧产业管理站；

【摘要】 【目的】探讨甘草酸二钾体外抗MDV的活性及作用机制。【方法】在体外以鸡胚成纤维细胞为MDV增殖模型,利用MTT比色法、噬斑减少法通过抗病毒试验、病毒复制抑制试验、直接灭活试验来评价甘草酸二钾的抗病毒效果;并通过细胞凋亡试验解释其抗病毒机制。【结果】抗病毒试验结果表明,在安全浓度范围内,甘草酸二钾在体外具有较强的抗MDV的活性,其EC₅₀为893.5±36.99μg/mL,SI>3.36,最大抑制率达94.4%;在病毒复制抑制试验中,甘草酸二钾可以抑制病毒复制的整个过程:病毒直接灭活试验表明甘草酸二钾可直接杀灭病毒粒子且不呈时间依赖性:细胞凋亡试验结果显示甘草酸二钾能够诱导感染MDV的鸡胚成纤维细胞凋亡。【结论】甘草酸二钾在体外具有抗MDV的活性,可以通过诱导感染病毒的鸡胚成纤维细胞凋亡发挥抗病毒作用。更多还原

【Abstract】 【Objective】The aim of the study is to test the antiviral activity and mechanism of Dipotassium Glycyrrhizinate(DG) against Marek’s disease virus(MDV) in vitro.【Method】Using MTT test and standard plaque reduction assay on Chick Embryo Fibroblast cells(CEFs),the antiviral effect of DG against MDV was observed by antiviral assay,time-of-addition assay and virucidal assay,the antiviral mechanism was tested by cell apoptosis assay.【Result】Antiviral assay showed that DG could reduce the damage of MDV to CEFs,EC50 is was 893.5±36.99μg/mL,SI>3.36 and the inhibition ratio was 94.4%, at the maximum with no-cytotoxic concentration.Time-of-addition assay and vimcidal assay demonstrated that DG could inhibit the viral replication in the full replication cycle and inactivated MDV particles in non time-dependent manner.Moreover,DG could promot MDV-infected cells the apoptosis in vitro.【Conclusion】DG could against MDV on CEFs by promoting MDV-infected cells apoptosis in vitro.更多还原