【作者】 赵祎镭； 王晨； 杜智敏；

【Author】 ZHAO Yi-lei,WANG Chen,DU Zhi-min(Pharmacy college,Harbin Medical University,Harbin,150086)

【机构】 哈尔滨医科大学药学院；

【摘要】 目的探讨胆碱对小鼠心肌肥厚的保护作用及其机制。方法经胸主动脉缩窄法建立小鼠心肌肥厚模型。造模2天后,分别给予胆碱(14mg.kg-1.d-1,ip)和4-DAMP(7μg.kg-1.d-1,ip)干预,3周后观察小鼠左心室重量和心脏功能的变化,分析心肌细胞形态的改变,采用免疫印迹法测定心肌肥厚标志物β-MHC的蛋白表达,逆转录-聚合酶链反应检测miR-133a以及β-MHC的mRNA表达水平。结果胆碱能够明显减轻小鼠左心室重量,降低β-MHC蛋白和mRNA水平的表达,恢复紊乱的miR-133a表达水平。4-DAMP能逆转胆碱作用,使miR-133a表达水平再次降低。结论胆碱能够减轻胸主动脉缩窄诱导的小鼠心肌肥厚,其对心脏的保护作用可能与调控miR-133a的表达有关。更多还原

【Abstract】 OBJECTIVE To investigate the protective effect of choline on pathological cardiac hypertrophyand to explore its potential mechanisms.METHODS Cardiac hypertrophy was induced by transverse aortaconstriction(TAC) in mice.After modeling two days,Choline(14 mg.kg-1.d-1,i.p.) and 4-DAMP(7 μg.kg-1.d-1 i.p.)were used to explore the effects of M3 receptor on the cardiac hypertrophy.Three weeks later,left heart weight,cardiac function and morphological of myocardial cells were measured and analysis.Western blot was used todetermine the expression of β-MHC protein.RT-PCR was used to detect β-MHC mRNA and miR-133a level.RESULTS Administration of choline significantly attenuated hypertrophic response,as identified by decreasingleft heart weight,mRNA and protein levels of β-MHC and recovering miR-133a level.The expression miR-133awas reversed by 4-DAMP,the miR-133a level degraded again.CONCLUTIONS Our study for the first timeconfirmed that choline could benefit impaired cardiac functions in pressure overload-induced pathologicalhypertrophy by regulating miR-133a.更多还原